Helicobacter pylori cagA promoter region sequences influence CagA expression and interleukin 8 secretion

Rui M. Ferreira; Ines Pinto-Ribeiro; Xiaogang Wen; Ricardo Marcos-Pinto; Mário Dinis-Ribeiro; Fátima Carneiro; Ceu Figueiredo

doi:10.1093/infdis/jiv467

Helicobacter pylori cagA promoter region sequences influence CagA expression and interleukin 8 secretion

Rui M. Ferreira, Ines Pinto-Ribeiro, Xiaogang Wen, Ricardo Marcos-Pinto, Mário Dinis-Ribeiro, Fátima Carneiro, Ceu Figueiredo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized the cagA promoter and expression in 46 H. pylori strains from Portugal. Our results confirm the relationship between cagA promoter region variation and protein expression originally observed in strains from Colombia. We observed that individuals with intestinal metaplasia were all infected with H. pylori strains containing a specific cagA motif. Additionally, we provided novel functional evidence that strain-specific sequences in the cagA promoter region and CagA expression levels influence interleukin 8 secretion by the host gastric epithelial cells.

Original language	English
Pages (from-to)	669-673
Number of pages	5
Journal	The Journal of infectious diseases
Volume	213
Issue number	4
DOIs	https://doi.org/10.1093/infdis/jiv467
Publication status	Published - 15 Feb 2016
Externally published	Yes

Keywords

CagA expression
cagA promoter region
Helicobacter pylori
IL-8

Access to Document

10.1093/infdis/jiv467Licence: Other

Cite this

@article{32195602b9f842c39ecd186aecc9b3b5,

title = "Helicobacter pylori cagA promoter region sequences influence CagA expression and interleukin 8 secretion",

abstract = "Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized the cagA promoter and expression in 46 H. pylori strains from Portugal. Our results confirm the relationship between cagA promoter region variation and protein expression originally observed in strains from Colombia. We observed that individuals with intestinal metaplasia were all infected with H. pylori strains containing a specific cagA motif. Additionally, we provided novel functional evidence that strain-specific sequences in the cagA promoter region and CagA expression levels influence interleukin 8 secretion by the host gastric epithelial cells.",

keywords = "CagA expression, cagA promoter region, Helicobacter pylori, IL-8",

author = "Ferreira, {Rui M.} and Ines Pinto-Ribeiro and Xiaogang Wen and Ricardo Marcos-Pinto and M{\'a}rio Dinis-Ribeiro and F{\'a}tima Carneiro and Ceu Figueiredo",

note = "Publisher Copyright: {\textcopyright} The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America.",

year = "2016",

month = feb,

day = "15",

doi = "10.1093/infdis/jiv467",

language = "English",

volume = "213",

pages = "669--673",

journal = "The Journal of infectious diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "4",

}

TY - JOUR

T1 - Helicobacter pylori cagA promoter region sequences influence CagA expression and interleukin 8 secretion

AU - Ferreira, Rui M.

AU - Pinto-Ribeiro, Ines

AU - Wen, Xiaogang

AU - Marcos-Pinto, Ricardo

AU - Dinis-Ribeiro, Mário

AU - Carneiro, Fátima

AU - Figueiredo, Ceu

N1 - Publisher Copyright: © The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America.

PY - 2016/2/15

Y1 - 2016/2/15

N2 - Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized the cagA promoter and expression in 46 H. pylori strains from Portugal. Our results confirm the relationship between cagA promoter region variation and protein expression originally observed in strains from Colombia. We observed that individuals with intestinal metaplasia were all infected with H. pylori strains containing a specific cagA motif. Additionally, we provided novel functional evidence that strain-specific sequences in the cagA promoter region and CagA expression levels influence interleukin 8 secretion by the host gastric epithelial cells.

AB - Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized the cagA promoter and expression in 46 H. pylori strains from Portugal. Our results confirm the relationship between cagA promoter region variation and protein expression originally observed in strains from Colombia. We observed that individuals with intestinal metaplasia were all infected with H. pylori strains containing a specific cagA motif. Additionally, we provided novel functional evidence that strain-specific sequences in the cagA promoter region and CagA expression levels influence interleukin 8 secretion by the host gastric epithelial cells.

KW - CagA expression

KW - cagA promoter region

KW - Helicobacter pylori

KW - IL-8

UR - http://www.scopus.com/inward/record.url?scp=84960172758&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiv467

DO - 10.1093/infdis/jiv467

M3 - Article

C2 - 26401027

SN - 0022-1899

VL - 213

SP - 669

EP - 673

JO - The Journal of infectious diseases

JF - The Journal of infectious diseases

IS - 4

ER -

Helicobacter pylori cagA promoter region sequences influence CagA expression and interleukin 8 secretion

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this