Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain

João R. Mesquita; Danielle Oliveira; Enrique Rivadulla; Joana Abreu-Silva; Miguel F. Varela; Jesús L. Romalde; Maria S. J. Nascimento

doi:10.1016/j.fm.2016.03.009

Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain

João R. Mesquita, Danielle Oliveira, Enrique Rivadulla, Joana Abreu-Silva, Miguel F. Varela, Jesús L. Romalde^*, Maria S. J. Nascimento

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.

Original language	English
Pages (from-to)	13-15
Number of pages	3
Journal	Food Microbiology
Volume	58
DOIs	https://doi.org/10.1016/j.fm.2016.03.009
Publication status	Published - 1 Sept 2016
Externally published	Yes

Keywords

Detection
Hepatitis E virus
Shellfish

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.fm.2016.03.009

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title = "Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain",

abstract = "Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.",

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author = "Mesquita, {Jo{\~a}o R.} and Danielle Oliveira and Enrique Rivadulla and Joana Abreu-Silva and Varela, {Miguel F.} and Romalde, {Jes{\'u}s L.} and Nascimento, {Maria S. J.}",

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doi = "10.1016/j.fm.2016.03.009",

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AU - Mesquita, João R.

AU - Oliveira, Danielle

AU - Rivadulla, Enrique

AU - Abreu-Silva, Joana

AU - Varela, Miguel F.

AU - Romalde, Jesús L.

AU - Nascimento, Maria S. J.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.

AB - Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.

KW - Detection

KW - Hepatitis E virus

KW - Shellfish

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M3 - Article

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AN - SCOPUS:84961223568

SN - 0740-0020

VL - 58

SP - 13

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JO - Food Microbiology

JF - Food Microbiology

ER -

Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain

Abstract

Keywords

UN SDGs

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