TY - JOUR
T1 - High performance liquid chromatography coupled to an optical fiber detector coated with laccase for screening catecholamines in plasma and urine
AU - Ferreira, Filipe D.P.
AU - Silva, Lurdes I.B.
AU - Freitas, A. C.
AU - Rocha-Santos, Teresa A. P.
AU - Duarte, A. C.
PY - 2009/10/16
Y1 - 2009/10/16
N2 - An analytical method based on separation by high performance liquid chromatography (HPLC) and detection by optical fiber (OF) coated with an enzyme (laccase), has been developed for separation and quantification of catecholamines, namely epinephrine, dopamine and norepinephrine. The application of OF as a detector in this analytical system relies on the variation of the reflected optical power detected when the catecholamines eluted from the HPLC column act as the substrate of the laccase immobilized on a tip of a single-mode OF. The developed method shows a high linearity in a range between 5 and 125 pg/mL and detection limits of 3.5, 2.9 and 3.3 pg/mL for epinephrine, dopamine and norepinephrine, respectively. The analytical performance of the proposed method was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED) regarding catecholamines detection, showing great analytical advantages such as low cost of equipment. Additionally, the proposed method was applied to catecholamines determination in actual samples of plasma and human urine.
AB - An analytical method based on separation by high performance liquid chromatography (HPLC) and detection by optical fiber (OF) coated with an enzyme (laccase), has been developed for separation and quantification of catecholamines, namely epinephrine, dopamine and norepinephrine. The application of OF as a detector in this analytical system relies on the variation of the reflected optical power detected when the catecholamines eluted from the HPLC column act as the substrate of the laccase immobilized on a tip of a single-mode OF. The developed method shows a high linearity in a range between 5 and 125 pg/mL and detection limits of 3.5, 2.9 and 3.3 pg/mL for epinephrine, dopamine and norepinephrine, respectively. The analytical performance of the proposed method was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED) regarding catecholamines detection, showing great analytical advantages such as low cost of equipment. Additionally, the proposed method was applied to catecholamines determination in actual samples of plasma and human urine.
KW - Catecholamines
KW - HPLC
KW - Laccase
KW - Optical fiber detector
KW - Plasma
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=70349239100&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2009.08.067
DO - 10.1016/j.chroma.2009.08.067
M3 - Article
C2 - 19747685
AN - SCOPUS:70349239100
SN - 0021-9673
VL - 1216
SP - 7049
EP - 7054
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 42
ER -