@article{4e080d93eaab4b06a4685fcbc6e2a3d8,
title = "Hospital discharges in urban sanitation systems: long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome",
abstract = "Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.",
keywords = "Antimicrobial resistance, Hospital and urban wastewater, Hospital and urban wastewater eco-exposome, Microbiota, Resistome (ARGs and MGEs), WW signatures",
author = "Elena Buelow and Andreu Rico and Margaux Gaschet and Jos{\'e} Louren{\c c}o and Kennedy, {Sean P.} and Laure Wiest and Ploy, {Marie Cecile} and Christophe Dagot",
note = "Funding Information: A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University ( IJCI-2017-33,465 ). Funding Information: J. Louren{\c c}o is supported by a Lectureship from the Department of Zoology, University of Oxford . Funding Information: E. Buelow has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement RESOLVE 707999-standard EF. Funding Information: E. Buelow has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement RESOLVE 707999-standard EF.A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University (IJCI-2017-33,465).J. Louren?o is supported by a Lectureship from the Department of Zoology, University of Oxford.16S rRNA sequence Data collection and analyses performed by the IBEST Genomics Resources Core at the University of Idaho were supported in part by NIH COBRE grant P30GM103324. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = may,
day = "1",
doi = "10.1016/j.wroa.2020.100045",
language = "English",
volume = "7",
journal = "Water Research X",
issn = "2589-9147",
publisher = "Elsevier Ltd.",
}
