Hospital discharges in urban sanitation systems: long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome

Elena Buelow; Andreu Rico; Margaux Gaschet; José Lourenço; Sean P. Kennedy; Laure Wiest; Marie Cecile Ploy; Christophe Dagot

doi:10.1016/j.wroa.2020.100045

Hospital discharges in urban sanitation systems: long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome

Elena Buelow^*
, Andreu Rico
, Margaux Gaschet
, José Lourenço
, Sean P. Kennedy
, Laure Wiest
, Marie Cecile Ploy
, Christophe Dagot

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.

Original language	English
Article number	100045
Journal	Water Research X
Volume	7
DOIs	https://doi.org/10.1016/j.wroa.2020.100045
Publication status	Published - 1 May 2020
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation
SDG 11 Sustainable Cities and Communities

Keywords

Antimicrobial resistance
Hospital and urban wastewater
Hospital and urban wastewater eco-exposome
Microbiota
Resistome (ARGs and MGEs)
WW signatures

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10.1016/j.wroa.2020.100045Licence: CC BY-NC-ND

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title = "Hospital discharges in urban sanitation systems: long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome",

abstract = "Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.",

keywords = "Antimicrobial resistance, Hospital and urban wastewater, Hospital and urban wastewater eco-exposome, Microbiota, Resistome (ARGs and MGEs), WW signatures",

author = "Elena Buelow and Andreu Rico and Margaux Gaschet and Jos{\'e} Louren{\c c}o and Kennedy, \{Sean P.\} and Laure Wiest and Ploy, \{Marie Cecile\} and Christophe Dagot",

note = "Funding Information: A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University ( IJCI-2017-33,465 ). Funding Information: J. Louren{\c c}o is supported by a Lectureship from the Department of Zoology, University of Oxford . Funding Information: E. Buelow has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement RESOLVE 707999-standard EF. Funding Information: E. Buelow has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement RESOLVE 707999-standard EF.A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University (IJCI-2017-33,465).J. Louren?o is supported by a Lectureship from the Department of Zoology, University of Oxford.16S rRNA sequence Data collection and analyses performed by the IBEST Genomics Resources Core at the University of Idaho were supported in part by NIH COBRE grant P30GM103324. Publisher Copyright: {\textcopyright} 2020 The Authors",

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doi = "10.1016/j.wroa.2020.100045",

language = "English",

volume = "7",

journal = "Water Research X",

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T1 - Hospital discharges in urban sanitation systems

T2 - long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome

AU - Buelow, Elena

AU - Rico, Andreu

AU - Gaschet, Margaux

AU - Lourenço, José

AU - Kennedy, Sean P.

AU - Wiest, Laure

AU - Ploy, Marie Cecile

AU - Dagot, Christophe

N1 - Funding Information: A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University ( IJCI-2017-33,465 ). Funding Information: J. Lourenço is supported by a Lectureship from the Department of Zoology, University of Oxford . Funding Information: E. Buelow has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement RESOLVE 707999-standard EF. Funding Information: E. Buelow has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement RESOLVE 707999-standard EF.A. Rico is supported by a postdoctoral grant provided by the Spanish Ministry of Science, Innovation and University (IJCI-2017-33,465).J. Louren?o is supported by a Lectureship from the Department of Zoology, University of Oxford.16S rRNA sequence Data collection and analyses performed by the IBEST Genomics Resources Core at the University of Idaho were supported in part by NIH COBRE grant P30GM103324. Publisher Copyright: © 2020 The Authors

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.

AB - Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into the environment. Hospital WW (HWW) contain higher loads of micro-pollutants and AMR markers than urban WW (UWW). Little is known about the long-term dynamics of H and U WW and the impact of their joined treatment on the general burden of AMR. Here, we characterized the resistome, microbiota and eco-exposome signature of 126 H and U WW samples treated separately for three years, and then mixed, over one year. Multi-variate analysis and machine learning revealed a robust signature for each WW with no significant variation over time before mixing, and once mixed, both WW closely resembled Urban signatures. We demonstrated a significant impact of pharmaceuticals and surfactants on the resistome and microbiota of H and U WW. Our results present considerable targets for AMR related risk assessment of WW.

KW - Antimicrobial resistance

KW - Hospital and urban wastewater

KW - Hospital and urban wastewater eco-exposome

KW - Microbiota

KW - Resistome (ARGs and MGEs)

KW - WW signatures

UR - https://www.scopus.com/pages/publications/85084639014

U2 - 10.1016/j.wroa.2020.100045

DO - 10.1016/j.wroa.2020.100045

M3 - Article

C2 - 32072151

AN - SCOPUS:85084639014

SN - 2589-9147

VL - 7

JO - Water Research X

JF - Water Research X

M1 - 100045

ER -

Hospital discharges in urban sanitation systems: long-term monitoring of wastewater resistome and microbiota in relationship to their eco-exposome

Abstract

UN SDGs

Keywords

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