How neutral red modified carbon and electron flow in clostridium acetobutylicum grown in chemostat culture at neutral pH

Laurence Girbal, Isabel Vasconcelos, Silvie Saint‐Amans, Philippe Soucaille*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

The metabolism of Clostridium acetobutylicum was manipulated, at neutral pH and in chemostat culture, by the addition of Neutral red, a molecule that can replace ferredoxin in the oxido‐reduction reactions catalysed by the enzymes involved in the distribution of the electron flow. Cultures grown on glucose alone produced mainly acids while cultures grown on glucose plus Neutral red produced mainly alcohols and butyrate and low levels of hydrogen. We demonstrated that just after addition of Neutral red to an acidogenic culture, the simultaneous utilizations of ferredoxin and dye deviate electron flow from hydrogen to NADH production initially by the enzymatic regulation of in vivo hydrogenase and ferredoxin NAD reductase activities. The higher NAD(P)H pool generated might, thereafter, be the signal for the setting up of a new metabolism. In the resulting steady‐state, the NAD(P)H ‘pressure’ is maintained by high ferredoxin NAD and NADP reductases level associated to a low NADH ferredoxin reductase level. The regeneration of NAD is mainly achieved via the induced or increased NADH‐dependent aldehyde and alcohol dehydrogenase activities.
Original languageEnglish
Pages (from-to)151-162
Number of pages12
JournalFEMS Microbiology Reviews
Volume16
Issue number2-3
DOIs
Publication statusPublished - Feb 1995
Externally publishedYes

Keywords

  • Alcohol shift
  • Clostridium acetobutylicum
  • Ferredoxin NAD oxidoreductase
  • Hydrogenase
  • NADH
  • Neutral red
  • Pyruvate ferredoxin oxidoreductase

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