Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells

Sara Amorim; Diana Soares da Costa; Iva Pashkuleva; Celso A. Reis; Rui L. Reis; Ricardo A. Pires

doi:10.1002/adbi.202000122

Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells

Sara Amorim^*, Diana Soares da Costa, Iva Pashkuleva, Celso A. Reis, Rui L. Reis, Ricardo A. Pires^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layer-by-layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HA-rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the “hummingbird” morphology, promote high cellular motility, and activate signaling pathways with increased expression of p-ERK1/2 and p-AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.

Original language	English
Article number	2000122
Number of pages	7
Journal	Advanced Biosystems
Volume	4
Issue number	11
DOIs	https://doi.org/10.1002/adbi.202000122
Publication status	Published - Nov 2020
Externally published	Yes

Keywords

Cancer invasiveness
Hummingbird
Hyaluronic acid
Molecular weight

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/adbi.202000122

Cite this

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title = "Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells",

abstract = "The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layer-by-layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HA-rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the “hummingbird” morphology, promote high cellular motility, and activate signaling pathways with increased expression of p-ERK1/2 and p-AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.",

keywords = "Cancer invasiveness, Hummingbird, Hyaluronic acid, Molecular weight",

author = "Sara Amorim and Costa, {Diana Soares da} and Iva Pashkuleva and Reis, {Celso A.} and Reis, {Rui L.} and Pires, {Ricardo A.}",

note = "Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",

year = "2020",

month = nov,

doi = "10.1002/adbi.202000122",

language = "English",

volume = "4",

journal = "Advanced Biosystems",

issn = "2366-7478",

publisher = "Wiley-VCH Verlag",

number = "11",

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TY - JOUR

T1 - Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells

AU - Amorim, Sara

AU - Costa, Diana Soares da

AU - Pashkuleva, Iva

AU - Reis, Celso A.

AU - Reis, Rui L.

AU - Pires, Ricardo A.

PY - 2020/11

Y1 - 2020/11

N2 - The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layer-by-layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HA-rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the “hummingbird” morphology, promote high cellular motility, and activate signaling pathways with increased expression of p-ERK1/2 and p-AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.

AB - The overproduction and deposition of hyaluronic acid (HA) of different sizes in the tumor microenvironment is associated with cancer metastasis. Here, the development of layer-by-layer (LbL) constructs containing HA of different molecular weights (i.e., 5.6, 618, and 1450 kDa) that mimic the HA-rich cancer extracellular matrix is described to study the effect of the HA's size on the behavior of gastric cancer cells (AGS). The results demonstrate that LbL constructs with short HA, i.e., 5.6 kDa, activate the cytoskeleton rearrangement leading to the “hummingbird” morphology, promote high cellular motility, and activate signaling pathways with increased expression of p-ERK1/2 and p-AKT. In addition, it is demonstrated that this malignant transformation involves an active participation of the HA coreceptor RHAMM in AGS cells.

KW - Cancer invasiveness

KW - Hummingbird

KW - Hyaluronic acid

KW - Molecular weight

UR - http://www.scopus.com/inward/record.url?scp=85092021672&partnerID=8YFLogxK

U2 - 10.1002/adbi.202000122

DO - 10.1002/adbi.202000122

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SN - 2366-7478

VL - 4

JO - Advanced Biosystems

JF - Advanced Biosystems

IS - 11

M1 - 2000122

ER -

Hyaluronic acid of low molecular weight triggers the invasive “hummingbird” phenotype on gastric cancer cells

Abstract

Keywords

UN SDGs

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