Hydrolysis of caseins by extracts of Cynara cardunculus precipitated by ammonium sulfate

S. V. Silva; R. M. Barros; F. X. Malcata

doi:10.1111/j.1365-2621.2002.tb08717.x

Hydrolysis of caseins by extracts of Cynara cardunculus precipitated by ammonium sulfate

S. V. Silva, R. M. Barros, F. X. Malcata^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.

Original language	English
Pages (from-to)	1746-1751
Number of pages	6
Journal	Journal of Food Science
Volume	67
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2621.2002.tb08717.x
Publication status	Published - 2002

Keywords

FPLC
Milk proteins
Thistle
Urea-PAGE

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10.1111/j.1365-2621.2002.tb08717.x

http://hdl.handle.net/10400.14/6808

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title = "Hydrolysis of caseins by extracts of Cynara cardunculus precipitated by ammonium sulfate",

abstract = "Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.",

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AU - Silva, S. V.

AU - Barros, R. M.

AU - Malcata, F. X.

PY - 2002

Y1 - 2002

N2 - Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.

AB - Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.

KW - FPLC

KW - Milk proteins

KW - Thistle

KW - Urea-PAGE

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JO - Journal of Food Science

JF - Journal of Food Science

IS - 5

ER -

Hydrolysis of caseins by extracts of Cynara cardunculus precipitated by ammonium sulfate

Abstract

Keywords

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