TY - JOUR
T1 - Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy
AU - Sousa, Clara
AU - Grosso, Filipa
AU - Meirinhos-Soares, Luís
AU - Peixe, Luísa
AU - Lopes, João
PY - 2014/5
Y1 - 2014/5
N2 - In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D β -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58, ST98 carrying blaOXA-24/40and ST92 carrying blaOXA-23. Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates.
AB - In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D β -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58, ST98 carrying blaOXA-24/40and ST92 carrying blaOXA-23. Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates.
KW - Acinetobacter baumannii
KW - Carbapenems
KW - Chemometrics
KW - Infrared spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=84899677143&partnerID=8YFLogxK
U2 - 10.1002/jbio.201200075
DO - 10.1002/jbio.201200075
M3 - Article
C2 - 23192934
AN - SCOPUS:84899677143
SN - 1864-063X
VL - 7
SP - 287
EP - 294
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 5
ER -