In situ CLA and CLNA production: a potential strategy to elaborate food products enriched in bioactive fatty acids

L. L. Pimentel; A. L. Fontes; A. Gomes; L. M. Rodríguez-Alcalá

Abstract

Conjugated linoleic acid (CLA) and, more recently, conjugated linolenic acid (CLNA) isomers have shown potential to be applied as new functional ingredients, given its bioactive potential to exert benefits to human health. These fatty acids are naturally produced by rumen bacteria that convert linoleic acid (LA) and linolenic acid (LNA) into their conjugated forms. However, strains of lactobacilli, bifidobacteria and propionibacteria have also revealed capacity to produce these compounds. Thus, the aim of this work was to test the in situ production of CLA and CLNA isomers in a dairy matrix using a potential producing strain, in order to verify the suitability of this strategy for CLA and CLNA enrichment in food products. Bifidobacterium breve NCIMB 702258 was selected for this study as previously showed CLA/CLnA production capacity. Its CLA/CLNA-producing ability was firstly tested by incubation in MRS medium containing LA, -LNA or both (at 0.5 mg/mL each) for 24 h at 37 ºC. Fatty acid concentration was analyzed in the supernatant through gas chromatography and it was revealed LA and -LNA conversion rates of, respectively, 30.36% and 67.33%, when substrates were added separately, and of 14.83% and 55.40%, when together, being CLNA production higher than CLA in both situations. Bifidobacterium breve producing capacity was then tested in a food matrix (semi- skimmed milk, 1.6% fat) under the same conditions applied in MRS medium. CLA/CLNA isomers production was positive although substrate conversion rates were lower than those obtained in MRS medium. -LNA conversion was higher than LA when the substrates were added separately (28.09% and 24.19%, respectively), but not when both LA and -LNA were assayed together (14.55% and 27.60%, respectively). In conclusion, in situ production of microbial CLA and CLNA isomers is a strategy with potential to be applied in the future elaboration of CLA- and CLNA-enriched food products.

Original language	English
Pages	1-1
Number of pages	1
Publication status	Published - 2017
Event	15th Euro Fed Lipid Congress - Upsalla, Sweden Duration: 27 Aug 2017 → 30 Aug 2017

Conference

Conference	15th Euro Fed Lipid Congress
Country/Territory	Sweden
City	Upsalla
Period	27/08/17 → 30/08/17

Access to Document

108538836Final published version, 30.9 KBLicence: Unspecified

http://hdl.handle.net/10400.14/47350Licence: Unspecified

Cite this

@conference{8c965634f4424aea9c9f11365f32a85f,

title = "In situ CLA and CLNA production: a potential strategy to elaborate food products enriched in bioactive fatty acids",

abstract = "Conjugated linoleic acid (CLA) and, more recently, conjugated linolenic acid (CLNA) isomers have shown potential to be applied as new functional ingredients, given its bioactive potential to exert benefits to human health. These fatty acids are naturally produced by rumen bacteria that convert linoleic acid (LA) and linolenic acid (LNA) into their conjugated forms. However, strains of lactobacilli, bifidobacteria and propionibacteria have also revealed capacity to produce these compounds. Thus, the aim of this work was to test the in situ production of CLA and CLNA isomers in a dairy matrix using a potential producing strain, in order to verify the suitability of this strategy for CLA and CLNA enrichment in food products. Bifidobacterium breve NCIMB 702258 was selected for this study as previously showed CLA/CLnA production capacity. Its CLA/CLNA-producing ability was firstly tested by incubation in MRS medium containing LA, -LNA or both (at 0.5 mg/mL each) for 24 h at 37 ºC. Fatty acid concentration was analyzed in the supernatant through gas chromatography and it was revealed LA and -LNA conversion rates of, respectively, 30.36% and 67.33%, when substrates were added separately, and of 14.83% and 55.40%, when together, being CLNA production higher than CLA in both situations. Bifidobacterium breve producing capacity was then tested in a food matrix (semi- skimmed milk, 1.6% fat) under the same conditions applied in MRS medium. CLA/CLNA isomers production was positive although substrate conversion rates were lower than those obtained in MRS medium. -LNA conversion was higher than LA when the substrates were added separately (28.09% and 24.19%, respectively), but not when both LA and -LNA were assayed together (14.55% and 27.60%, respectively). In conclusion, in situ production of microbial CLA and CLNA isomers is a strategy with potential to be applied in the future elaboration of CLA- and CLNA-enriched food products.",

author = "Pimentel, {L. L.} and Fontes, {A. L.} and A. Gomes and Rodr{\'i}guez-Alcal{\'a}, {L. M.}",

year = "2017",

language = "English",

pages = "1--1",

note = "15th Euro Fed Lipid Congress ; Conference date: 27-08-2017 Through 30-08-2017",

}

TY - CONF

T1 - In situ CLA and CLNA production

T2 - 15th Euro Fed Lipid Congress

AU - Pimentel, L. L.

AU - Fontes, A. L.

AU - Gomes, A.

AU - Rodríguez-Alcalá, L. M.

PY - 2017

Y1 - 2017

N2 - Conjugated linoleic acid (CLA) and, more recently, conjugated linolenic acid (CLNA) isomers have shown potential to be applied as new functional ingredients, given its bioactive potential to exert benefits to human health. These fatty acids are naturally produced by rumen bacteria that convert linoleic acid (LA) and linolenic acid (LNA) into their conjugated forms. However, strains of lactobacilli, bifidobacteria and propionibacteria have also revealed capacity to produce these compounds. Thus, the aim of this work was to test the in situ production of CLA and CLNA isomers in a dairy matrix using a potential producing strain, in order to verify the suitability of this strategy for CLA and CLNA enrichment in food products. Bifidobacterium breve NCIMB 702258 was selected for this study as previously showed CLA/CLnA production capacity. Its CLA/CLNA-producing ability was firstly tested by incubation in MRS medium containing LA, -LNA or both (at 0.5 mg/mL each) for 24 h at 37 ºC. Fatty acid concentration was analyzed in the supernatant through gas chromatography and it was revealed LA and -LNA conversion rates of, respectively, 30.36% and 67.33%, when substrates were added separately, and of 14.83% and 55.40%, when together, being CLNA production higher than CLA in both situations. Bifidobacterium breve producing capacity was then tested in a food matrix (semi- skimmed milk, 1.6% fat) under the same conditions applied in MRS medium. CLA/CLNA isomers production was positive although substrate conversion rates were lower than those obtained in MRS medium. -LNA conversion was higher than LA when the substrates were added separately (28.09% and 24.19%, respectively), but not when both LA and -LNA were assayed together (14.55% and 27.60%, respectively). In conclusion, in situ production of microbial CLA and CLNA isomers is a strategy with potential to be applied in the future elaboration of CLA- and CLNA-enriched food products.

AB - Conjugated linoleic acid (CLA) and, more recently, conjugated linolenic acid (CLNA) isomers have shown potential to be applied as new functional ingredients, given its bioactive potential to exert benefits to human health. These fatty acids are naturally produced by rumen bacteria that convert linoleic acid (LA) and linolenic acid (LNA) into their conjugated forms. However, strains of lactobacilli, bifidobacteria and propionibacteria have also revealed capacity to produce these compounds. Thus, the aim of this work was to test the in situ production of CLA and CLNA isomers in a dairy matrix using a potential producing strain, in order to verify the suitability of this strategy for CLA and CLNA enrichment in food products. Bifidobacterium breve NCIMB 702258 was selected for this study as previously showed CLA/CLnA production capacity. Its CLA/CLNA-producing ability was firstly tested by incubation in MRS medium containing LA, -LNA or both (at 0.5 mg/mL each) for 24 h at 37 ºC. Fatty acid concentration was analyzed in the supernatant through gas chromatography and it was revealed LA and -LNA conversion rates of, respectively, 30.36% and 67.33%, when substrates were added separately, and of 14.83% and 55.40%, when together, being CLNA production higher than CLA in both situations. Bifidobacterium breve producing capacity was then tested in a food matrix (semi- skimmed milk, 1.6% fat) under the same conditions applied in MRS medium. CLA/CLNA isomers production was positive although substrate conversion rates were lower than those obtained in MRS medium. -LNA conversion was higher than LA when the substrates were added separately (28.09% and 24.19%, respectively), but not when both LA and -LNA were assayed together (14.55% and 27.60%, respectively). In conclusion, in situ production of microbial CLA and CLNA isomers is a strategy with potential to be applied in the future elaboration of CLA- and CLNA-enriched food products.

M3 - Abstract

SP - 1

EP - 1

Y2 - 27 August 2017 through 30 August 2017

ER -

In situ CLA and CLNA production: a potential strategy to elaborate food products enriched in bioactive fatty acids

Abstract

Conference

Access to Document

Fingerprint

Cite this