In situ crosslinked electrospun gelatin nanofibers for skin regeneration

J. R. Dias*, S. Baptista-Silva, C. M. T. de Oliveira, A. Sousa, A. L. Oliveira, P. J. Bártolo, P. L. Granja

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Due to its intrinsic similarity to the extracellular matrix, gelatin electrospun nanofibrous meshes are promising scaffold structures for wound dressings and tissue engineering applications. However, gelatin is water soluble and presents poor mechanical properties, which generally constitute relevant limitations to its applicability. In this work, gelatin was in situ crosslinked with 1,4-butanediol diglycidyl ether (BDDGE) at different concentrations (2, 4 and 6 wt%) and incubation time-points (24, 48 and 72 h) at 37 °C. The physico-chemical and biological properties of BDDGE-crosslinked electrospun gelatin meshes were investigated. Results show that by changing the BDDGE concentration it is possible to produce nanofibers crosslinked in situ with well-defined morphology and modulate fiber size and mechanical properties. Crosslinked gelatin meshes show no toxicity towards fibroblasts, stimulating their adhesion, proliferation and synthesis of new extracellular matrix, thereby indicating the potential of this strategy for skin tissue engineering.

Original languageEnglish
Pages (from-to)161-173
Number of pages13
JournalEuropean Polymer Journal
Volume95
DOIs
Publication statusPublished - Oct 2017

Keywords

  • BDDGE
  • Electrospun fibers
  • Gelatin
  • In situ crosslinking
  • Wound dressing

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