TY - JOUR
T1 - In vitro evaluation of "horchata" co-products as carbon source for probiotic bacteria growth
AU - Sánchez-Zapata, Elena
AU - Fernández-López, Juana
AU - Pérez-Alvarez, José A.
AU - Soares, José
AU - Sousa, Sérgio
AU - Gomes, Ana M. P.
AU - Pintado, Manuela M. E.
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China ( 21571179 ), National Key R&D Program of China ( 2017YFE0106900 ), “Hundreds Talents Program” and Science and Technology Service Network Initiative from Chinese Academy of Sciences and Xiamen Technology Plan ( 3502Z20172032 ).
PY - 2013/7/1
Y1 - 2013/7/1
N2 - Tiger nut milk ("horchata") liquid co-products (TNLC) were evaluated as carbon source for probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis) growth by screening via microplate assay and determination of viable cells and metabolic activity. Based on MRS five different basal media were prepared ((i) without carbon source, (ii) with 2% (w/v) glucose, (iii) with 2% (w/v) FOS, (iv) with 2% (v/v) TNLC and (v) 3% (v/v) TNLC). Additionally, reconstituted TNLC was also used as basal medium. For determination of viable cells and metabolic activity skim milk powder was used. Glucose was found to be the best substrate to L. acidophilus grows, followed by FOS and TNLC (2% and 3%, respectively). TNLC (3%) was found to be the best substrate followed by TNLC (2%), glucose and FOS, in the promotion of growth of B. animalis. The growth of L. acidophilus and B. animalis in skim milk reconstituted TNLC + water was higher than samples inoculated in skim milk reconstituted only with water (p < 0.05). This is supported by the pH effect and by the faster organic acid production (mainly lactic acid, acetic acid, and butyric acid), confirming the property of TNLC as a carbon source for probiotic bacteria growth.
AB - Tiger nut milk ("horchata") liquid co-products (TNLC) were evaluated as carbon source for probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis) growth by screening via microplate assay and determination of viable cells and metabolic activity. Based on MRS five different basal media were prepared ((i) without carbon source, (ii) with 2% (w/v) glucose, (iii) with 2% (w/v) FOS, (iv) with 2% (v/v) TNLC and (v) 3% (v/v) TNLC). Additionally, reconstituted TNLC was also used as basal medium. For determination of viable cells and metabolic activity skim milk powder was used. Glucose was found to be the best substrate to L. acidophilus grows, followed by FOS and TNLC (2% and 3%, respectively). TNLC (3%) was found to be the best substrate followed by TNLC (2%), glucose and FOS, in the promotion of growth of B. animalis. The growth of L. acidophilus and B. animalis in skim milk reconstituted TNLC + water was higher than samples inoculated in skim milk reconstituted only with water (p < 0.05). This is supported by the pH effect and by the faster organic acid production (mainly lactic acid, acetic acid, and butyric acid), confirming the property of TNLC as a carbon source for probiotic bacteria growth.
KW - Bifidobacterium animalis
KW - Lactobacillus acidophilus
KW - Microdilution assay
KW - Organic acids
KW - Tiger nut
UR - http://www.scopus.com/inward/record.url?scp=84878860626&partnerID=8YFLogxK
U2 - 10.1016/j.fbp.2012.11.003
DO - 10.1016/j.fbp.2012.11.003
M3 - Article
AN - SCOPUS:84878860626
SN - 0960-3085
VL - 91
SP - 279
EP - 286
JO - Food and Bioproducts Processing
JF - Food and Bioproducts Processing
IS - 3
ER -