Abstract
The prebiotic effect of oligosaccharides recovered and purified from caprine whey, was evaluated by in vitro fermentation under anaerobic conditions using batch cultures at 37°C with human faeces. Effects on key gut bacterial groups were monitored over 24 h by fluorescence in situ hybridization (FISH), which was used to determine a quantitative prebiotic index score. Production of short-chain fatty acids (SCFAs) as fermentation end products was analyzed by high-performance liquid chromatography (HPLC). Growth of Bifidobacterium spp was significantly higher (P ≥ 0.05) with the purified oligosaccharides compared to the negative control. Lactic and propionic acids were the main SCFAs produced. Antimicrobial activity of the oligosaccharides was also tested, revealing no inhibition though a decrease in Staphylococcus aureus and Escherichia coli growth. These findings indicate that naturally extracted oligosaccharides from caprine whey could be used as new and valuable source of prebiotics. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
Original language | English |
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Pages (from-to) | 440-449 |
Number of pages | 10 |
Journal | BioFactors |
Volume | 38 |
Issue number | 6 |
DOIs | |
Publication status | Published - Nov 2012 |
Externally published | Yes |
Keywords
- Antimicrobial activity
- Caprine whey
- Oligosaccharide
- Prebiotic
- Prebiotic index