Inactivation kinetics of Listeria innocua in thermosonicated kiwi juice

Sharayu Bhutkar*, Teresa R. S. Brandão, Cristina L. M. Silva, Fátima A. Miller

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review



In fruit juice processing, traditional heat treatments such as pasteurization are usually used. However, this processing method may often induce undesirable quality changes in fruit juices. Alternatively, thermosonication has been found to have a great potential in microbial inactivation and fruit juices’ quality retention. The objective of this study was to evaluate thermosonication and thermal treatments’ influence on the inactivation kinetics of L. innocua 2030c (a surrogate of the pathogenic Listeria monocytogenes) in kiwifruit (Actinidia deliciosa cv. Hayward) juice. The kiwi peel was manually removed with a peeler to prepare the juice, and then flesh was sliced with a stainless-steel knife. The juice was extracted using a domestic centrifuge. The obtained juice was artificially inoculated with L. innocua subculture (~109 CFU/mL). The kiwi juice was adjusted to a pH of 3.6 by adding Cantaloupe melon (Cucumis melo L. var. reticulatus) juice, a natural component. Thermosonication was carried out in a water bath coupled with an ultrasound homogenizer at a constant frequency of 20 kHz, 80% amplitude and discontinue pulsation (10s on, 5s off). The juice samples were submitted to thermosonication treatments at 45, 50 and 55°C for 15, 10 and 3 minutes, respectively. At the same temperatures, thermal treatments were performed as a control for 60, 25 and 10 minutes. Each experiment was repeated three times. The Weibull model was used to fit all L. innocua log-survival data, based on regression analysis. For thermal treatment at 45°C, the first decimal reduction time (δ) obtained was 23.31 ± 3.51 min, while with thermosonication, the value was significantly reduced to 3.19 ± 0.59 min. The same happens to the other temperatures, with δ for thermal treatments at 50 and 55°C being 5.06 ± 1.73 and 2.50 ± 0.70 min, whereas, with thermosonication, the δ decreased respectively to 1.47 ± 0.59 and 0.46 ± 0.21 min. These results proved the existence of a synergistic effect between temperature and ultrasounds, making it possible to apply mild heat treatment processes and improve the final product’s quality. Since thermosonication treatment was effective in L. innocua inactivation, this technology can be considered a successful alternative to fruit juices’ conventional thermal treatment
Original languageEnglish
Title of host publication6th International ISEKI-food conference
Subtitle of host publicationsustainable development goals in food systems: challenges and opportunities for the future: book of abstracts
EditorsMargarida Vieira, Paolla Pittia, Cristina L. M. Silva, Florence Dubois-Brissonnet, Rui Costa, Foteini Chrysanthopoulou
Place of PublicationVienna
PublisherISEKI Food Association
Number of pages1
ISBN (Electronic)9789899023529
Publication statusPublished - 2021
Event6th International ISEKI-Food Conference : Sustainable Development Goals in Food System: challenges and opportunities for the future - Online, Austria
Duration: 23 Jun 202125 Jun 2021


Conference6th International ISEKI-Food Conference
Abbreviated title ISEKI-Food 2021


  • Thermosonication
  • Kiwifruit juice
  • Thermal treatment
  • Inactivation kinetics
  • L. innocua


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