Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

Anouk F. Duque, Syed A. Hasan, Vânia S. Bessa, Maria F. Carvalho, Ghufrana Samin, Dick B. Janssen, Paula M. L. Castro*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

A pure bacterial culture able to utilize 2- fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2- FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3- fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume95
Issue number2
DOIs
Publication statusPublished - Jul 2012

Keywords

  • 2-Fluorophenol (2-FP)
  • Biodegradation
  • Luong model
  • Monooxygenase
  • Rhodococcus sp.

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