TY - JOUR
T1 - Lactobacillus mulieris sp. nov., a new species of Lactobacillus delbrueckii group
AU - Rocha, Joana
AU - Botelho, João
AU - Ksiezarek, Magdalena
AU - Perovic, Svetlana Ugarcina
AU - Machado, Miguel
AU - Carriço, João André
AU - Pimentel, Lígia L.
AU - Salsinha, Sofia
AU - Rodríguez-Alcalá, Luís M.
AU - Pintado, Manuela
AU - Ribeiro, Teresa G.
AU - Peixe, Luísa
PY - 2020
Y1 - 2020
N2 - One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus. Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii, respectively. The major fatty acids of strain c10Ua161MT were C18: 1ω9c (65.0%), C16: 0 (17.8%), and summed feature 8 (10.2%; comprising C18: 1ω7c, and/or C18: 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus, for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).
AB - One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus. Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii, respectively. The major fatty acids of strain c10Ua161MT were C18: 1ω9c (65.0%), C16: 0 (17.8%), and summed feature 8 (10.2%; comprising C18: 1ω7c, and/or C18: 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus, for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).
KW - 16S rRNA
KW - Fatty acid
KW - Genome
KW - PheS
KW - RpoA
UR - http://www.scopus.com/inward/record.url?scp=85082780033&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003901
DO - 10.1099/ijsem.0.003901
M3 - Article
C2 - 31951193
AN - SCOPUS:85082780033
SN - 1466-5026
VL - 70
SP - 1522
EP - 1527
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 3
ER -