Lactobacillus mulieris sp. nov., a new species of lactobacillus delbrueckii group

Joana Rocha, João Botelho, Magdalena Ksiezarek, Svetlana Ugarcina Perovic, Miguel Machado, João André Carriço, Lígia L. Pimentel, Sofia Salsinha, Luís M. Rodríguez-Alcalá, Manuela Pintado, Teresa G. Ribeiro, Luísa Peixe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus. Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii, respectively. The major fatty acids of strain c10Ua161MT were C18: 1ω9c (65.0%), C16: 0 (17.8%), and summed feature 8 (10.2%; comprising C18: 1ω7c, and/or C18: 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus, for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).
Original languageEnglish
Article number003901
Pages (from-to)1522-1527
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • 16S rRNA
  • Fatty acid
  • Genome
  • PheS
  • RpoA

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