LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region

Walid Zeghbib; Fares Boudjouan; João Carneiro; Ana L. S. Oliveira; Sérgio F. Sousa; Manuela Estevez Pintado; Asma Ourabah; Vitor Vasconcelos; Graciliana Lopes

doi:10.1016/j.foodchem.2024.140414

LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region

Walid Zeghbib, Fares Boudjouan, João Carneiro, Ana L. S. Oliveira, Sérgio F. Sousa, Manuela Estevez Pintado, Asma Ourabah, Vitor Vasconcelos, Graciliana Lopes^*

^*Corresponding author for this work

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Abstract

Opuntia plants are abundant but still underexplored edible resources of the Algerian region. This work chemically characterizes extracts of different parts of the fruit of the commercial Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. growing in Bejaia, and evaluates their anti-inflammatory potential through different cell and cell-free bioassays. The LC-ESI-UHR-QqTOF-MS/MS analysis enabled the identification of 18 compounds, with azelaic acid and 1-O-vanilloyl-β-D-glucose reported here for the first time. Aqueous extracts of seeds were the most effective in scavenging superoxide anion radical (IC₅₀ = 111.08 μg/mL) and presented the best anti-inflammatory potential in LPS-stimulated macrophages (IC₅₀ = 206.30 μg/mL). The pulp of O. stricta suggested potential for addressing post-inflammatory hyperpigmentation, with piscidic and eucomic acids predicted with the strongest binding affinity towards tyrosinase, exhibiting higher scoring values than the reference inhibitor kojic acid. This pioneer study brings valuable perspectives for the pharmacological, nutritional and economic valorization of the wild O. stricta for functional foods.

Original language	English
Article number	140414
Number of pages	13
Journal	Food Chemistry
Volume	460
DOIs	https://doi.org/10.1016/j.foodchem.2024.140414
Publication status	Published - 1 Dec 2024

Keywords

Docking study
Hyaluronidase
Nitric oxide
Prickly pears
RAW 264.7
Superoxide anion radical
Tyrosinase

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Zeghbib, W., Boudjouan, F., Carneiro, J., Oliveira, A. L. S., Sousa, S. F., Pintado, M. E., Ourabah, A., Vasconcelos, V., & Lopes, G. (2024). LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region. Food Chemistry , 460, Article 140414. https://doi.org/10.1016/j.foodchem.2024.140414

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title = "LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region",

abstract = "Opuntia plants are abundant but still underexplored edible resources of the Algerian region. This work chemically characterizes extracts of different parts of the fruit of the commercial Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. growing in Bejaia, and evaluates their anti-inflammatory potential through different cell and cell-free bioassays. The LC-ESI-UHR-QqTOF-MS/MS analysis enabled the identification of 18 compounds, with azelaic acid and 1-O-vanilloyl-β-D-glucose reported here for the first time. Aqueous extracts of seeds were the most effective in scavenging superoxide anion radical (IC50 = 111.08 μg/mL) and presented the best anti-inflammatory potential in LPS-stimulated macrophages (IC50 = 206.30 μg/mL). The pulp of O. stricta suggested potential for addressing post-inflammatory hyperpigmentation, with piscidic and eucomic acids predicted with the strongest binding affinity towards tyrosinase, exhibiting higher scoring values than the reference inhibitor kojic acid. This pioneer study brings valuable perspectives for the pharmacological, nutritional and economic valorization of the wild O. stricta for functional foods.",

keywords = "Docking study, Hyaluronidase, Nitric oxide, Prickly pears, RAW 264.7, Superoxide anion radical, Tyrosinase",

author = "Walid Zeghbib and Fares Boudjouan and Jo{\~a}o Carneiro and Oliveira, {Ana L. S.} and Sousa, {S{\'e}rgio F.} and Pintado, {Manuela Estevez} and Asma Ourabah and Vitor Vasconcelos and Graciliana Lopes",

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doi = "10.1016/j.foodchem.2024.140414",

language = "English",

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Zeghbib, W, Boudjouan, F, Carneiro, J, Oliveira, ALS, Sousa, SF, Pintado, ME, Ourabah, A, Vasconcelos, V & Lopes, G 2024, 'LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region', Food Chemistry , vol. 460, 140414. https://doi.org/10.1016/j.foodchem.2024.140414

TY - JOUR

T1 - LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region

AU - Zeghbib, Walid

AU - Boudjouan, Fares

AU - Carneiro, João

AU - Oliveira, Ana L. S.

AU - Sousa, Sérgio F.

AU - Pintado, Manuela Estevez

AU - Ourabah, Asma

AU - Vasconcelos, Vitor

AU - Lopes, Graciliana

PY - 2024/12/1

Y1 - 2024/12/1

N2 - Opuntia plants are abundant but still underexplored edible resources of the Algerian region. This work chemically characterizes extracts of different parts of the fruit of the commercial Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. growing in Bejaia, and evaluates their anti-inflammatory potential through different cell and cell-free bioassays. The LC-ESI-UHR-QqTOF-MS/MS analysis enabled the identification of 18 compounds, with azelaic acid and 1-O-vanilloyl-β-D-glucose reported here for the first time. Aqueous extracts of seeds were the most effective in scavenging superoxide anion radical (IC50 = 111.08 μg/mL) and presented the best anti-inflammatory potential in LPS-stimulated macrophages (IC50 = 206.30 μg/mL). The pulp of O. stricta suggested potential for addressing post-inflammatory hyperpigmentation, with piscidic and eucomic acids predicted with the strongest binding affinity towards tyrosinase, exhibiting higher scoring values than the reference inhibitor kojic acid. This pioneer study brings valuable perspectives for the pharmacological, nutritional and economic valorization of the wild O. stricta for functional foods.

AB - Opuntia plants are abundant but still underexplored edible resources of the Algerian region. This work chemically characterizes extracts of different parts of the fruit of the commercial Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. growing in Bejaia, and evaluates their anti-inflammatory potential through different cell and cell-free bioassays. The LC-ESI-UHR-QqTOF-MS/MS analysis enabled the identification of 18 compounds, with azelaic acid and 1-O-vanilloyl-β-D-glucose reported here for the first time. Aqueous extracts of seeds were the most effective in scavenging superoxide anion radical (IC50 = 111.08 μg/mL) and presented the best anti-inflammatory potential in LPS-stimulated macrophages (IC50 = 206.30 μg/mL). The pulp of O. stricta suggested potential for addressing post-inflammatory hyperpigmentation, with piscidic and eucomic acids predicted with the strongest binding affinity towards tyrosinase, exhibiting higher scoring values than the reference inhibitor kojic acid. This pioneer study brings valuable perspectives for the pharmacological, nutritional and economic valorization of the wild O. stricta for functional foods.

KW - Docking study

KW - Hyaluronidase

KW - Nitric oxide

KW - Prickly pears

KW - RAW 264.7

KW - Superoxide anion radical

KW - Tyrosinase

UR - http://www.scopus.com/inward/record.url?scp=85199809634&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2024.140414

DO - 10.1016/j.foodchem.2024.140414

M3 - Article

C2 - 39084103

AN - SCOPUS:85199809634

SN - 0308-8146

VL - 460

JO - Food Chemistry

JF - Food Chemistry

M1 - 140414

ER -

LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultivated Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. fruits from the Algerian region

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Keywords

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