Major lipid classes separation of buttermilk, and cows, goats and ewes milk by high performance liquid chromatography with an evaporative light scattering detector focused on the phospholipid fraction

L. M. Rodríguez-Alcalá, J. Fontecha*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

99 Citations (Scopus)

Abstract

An improved HPLC-ELSD method has been developed for the analysis of the lipid classes of buttermilk and milk from different species, focused in the phospholipids fraction without a prior fractionation step and in a single run. The total lipid profile analysis showed the major and minor lipid compounds as cholesterol esters, triacylglycerides, cholesterol, diacylglycerides, free fatty acids, monoacylglycerides, and also the polar compounds as glucosylceramide, lactosylceramide, phosphatidyl-ethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin and lysophosphatidylcholine. The identification and quantification of the different compounds, using calibration curves made with individual standards and the low coefficients of variation obtained in the inter- and intra-assays showed the suitability of the developed method. In this study, we optimized and validated a quantitative HPLC-ELSD method at a concentration level suitable for routine analysis of the major lipid classes in milk and dairy products.

Original languageEnglish
Pages (from-to)3063-3066
Number of pages4
JournalJournal of Chromatography A
Volume1217
Issue number18
DOIs
Publication statusPublished - Apr 2010
Externally publishedYes

Keywords

  • Evaporative light-scattering detection (ELSD-HPLC)
  • Lipid classes separation
  • Milk phospholipids

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