Abstract
The present invention relates to a method for producing active recombinant collagenase from Aeromonas hydrophila for use in processes where the digestion of collagens is required, for instance for medical use in the treatment of diseases involving excessive deposition of collagens and collagen-mediated pathologies. Besides clinical and research uses, microbial collagenases have high bioengineering potential in industry, for instance in the food industry for tenderising meat or in the tanning industry, where they contribute to the leather dyeing process. Thus, the present invention aims at providing a method for obtaining and purifying active recombinant collagenase from Aeromonas hydrophila, in which method collagen is digested by collagenase at concentrations that are lower than the cytotoxic concentration of collagenase.
| Translated title of the contribution | Method to produce a recombinant collagenase to digest collagens |
|---|---|
| Original language | Portuguese |
| Patent number | WO2015068134 |
| IPC | C12N9/52 |
| Priority date | 7/11/13 |
| Filing date | 7/11/14 |
| Publication status | Refused/Withdrawn - 30 Nov 2016 |
| Externally published | Yes |