Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells

Catalina Alvarez, Ana Claudia Campos, Jonathan C. Howard*, Joana Loureiro, Urs Benedikt Müller, Ana Lina Rodrigues

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and quantify these events in different combinations of the host and the parasite.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages371-409
Number of pages39
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2071
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Diaphragm-derived cells
  • Flow cytometry
  • IRG proteins
  • Immunofluorescence
  • Innate immunity
  • Interferon-γ
  • Mouse
  • Parasitophorous vacuole
  • Reactive necrosis
  • Toxoplasma gondii

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