TY - GEN
T1 - Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures
AU - Lopes, Marta B.
AU - Scholtz, Teresa
AU - Silva, Daniel
AU - Santos, Inês
AU - Silva, Tito
AU - Sampaio, Pedro
AU - Couto, Andreia
AU - Lopes, Vitor V.
AU - Calado, Cecília R. C.
PY - 2012
Y1 - 2012
N2 - An integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide significant help for future modelling and monitoring implementation. In a second stage, FTIR spectroscopy coupled with chemometrics, namely PLS regression, shows its potential as a high-throughput technique for simultaneously estimating the key variables involved in the plasmid production process by E. coli cultures run under distinct conditions. Finally, owing to online monitoring and process control, an NIR fibre optic probe and chemometrics provided promising results concerning the control of biomass and carbon sources in E. coli cultures.
AB - An integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide significant help for future modelling and monitoring implementation. In a second stage, FTIR spectroscopy coupled with chemometrics, namely PLS regression, shows its potential as a high-throughput technique for simultaneously estimating the key variables involved in the plasmid production process by E. coli cultures run under distinct conditions. Finally, owing to online monitoring and process control, an NIR fibre optic probe and chemometrics provided promising results concerning the control of biomass and carbon sources in E. coli cultures.
KW - Control
KW - Escherichia coli
KW - Fourier transform infrared
KW - Modelling
KW - Monitoring
KW - Near-infrared
KW - Plasmid
KW - spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=84869462873&partnerID=8YFLogxK
U2 - 10.1109/ENBENG.2012.6331370
DO - 10.1109/ENBENG.2012.6331370
M3 - Conference contribution
AN - SCOPUS:84869462873
SN - 9781467345248
T3 - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
BT - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
T2 - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
Y2 - 23 February 2012 through 25 February 2012
ER -