Modulation of the uptake of critical nutrients by breast cancer cells by lactate: impact on cell survival, proliferation and migration

Marta Guedes; João R. Araújo; Ana Correia-Branco; Inês Gregório; Fátima Martel; Elisa Keating

doi:10.1016/j.yexcr.2016.01.008

Modulation of the uptake of critical nutrients by breast cancer cells by lactate: impact on cell survival, proliferation and migration

Marta Guedes, João R. Araújo, Ana Correia-Branco, Inês Gregório, Fátima Martel, Elisa Keating^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

This work aimed to characterize the uptake of folate and glucose by breast cancer cells and to study the effect of lactate upon the transport of these nutrients and upon cell viability, proliferation and migration capacity.Data obtained showed that: a) MCF7 cells uptake 3H-folic acid (3H-FA) at physiological but not at acidic pH; b) T47D cells accumulate 3H-FA and 14C-5-methyltetrahydrofolate (14C-5-MTHF) more efficiently at acidic than at physiological pH; c) 3H-deoxyglucose (3H-DG) uptake by T47D cells is sodium-independent, inhibited by cytochalasin B (CYT B) and stimulated by insulin.Regarding the effect of lactate, in T47D cells, acute (26 min) and chronic (24 h) exposure to lactic acid (LA) stimulated 3H-FA uptake. Acute exposure to LA also stimulated 3H-DG uptake and chronic exposure to LA significantly stimulated T47D cell migratory capacity.In conclusion, the transport of folates is strikingly different in two phenotypically similar breast cancer cell lines: MCF7 and T47D cells. Additionally, lactate seems to act as a signaling molecule which increases the uptake of nutrients and promotes the migration capacity of T47D cells.

Original language	English
Pages (from-to)	111-122
Number of pages	12
Journal	Experimental Cell Research
Volume	341
Issue number	2
DOIs	https://doi.org/10.1016/j.yexcr.2016.01.008
Publication status	Published - 15 Feb 2016

Keywords

Breast cancer
Folate uptake
Glucose uptake
Lactate
Warburg effect

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.yexcr.2016.01.008

http://hdl.handle.net/10400.14/23080

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title = "Modulation of the uptake of critical nutrients by breast cancer cells by lactate: impact on cell survival, proliferation and migration",

abstract = "This work aimed to characterize the uptake of folate and glucose by breast cancer cells and to study the effect of lactate upon the transport of these nutrients and upon cell viability, proliferation and migration capacity.Data obtained showed that: a) MCF7 cells uptake 3H-folic acid (3H-FA) at physiological but not at acidic pH; b) T47D cells accumulate 3H-FA and 14C-5-methyltetrahydrofolate (14C-5-MTHF) more efficiently at acidic than at physiological pH; c) 3H-deoxyglucose (3H-DG) uptake by T47D cells is sodium-independent, inhibited by cytochalasin B (CYT B) and stimulated by insulin.Regarding the effect of lactate, in T47D cells, acute (26 min) and chronic (24 h) exposure to lactic acid (LA) stimulated 3H-FA uptake. Acute exposure to LA also stimulated 3H-DG uptake and chronic exposure to LA significantly stimulated T47D cell migratory capacity.In conclusion, the transport of folates is strikingly different in two phenotypically similar breast cancer cell lines: MCF7 and T47D cells. Additionally, lactate seems to act as a signaling molecule which increases the uptake of nutrients and promotes the migration capacity of T47D cells.",

keywords = "Breast cancer, Folate uptake, Glucose uptake, Lactate, Warburg effect",

author = "Marta Guedes and Ara{\'u}jo, {Jo{\~a}o R.} and Ana Correia-Branco and In{\^e}s Greg{\'o}rio and F{\'a}tima Martel and Elisa Keating",

note = "Funding Information: This work was supported by Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT), COMPETE, QREN and FEDER ( PTDC/SAU-OSM/102239/2008 ; SFRH/BPD/40170/2007 ) and by KLEAR Project (NORTE-07–0124-FEDER-000052), cofunded by FEDER and FCT. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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T2 - impact on cell survival, proliferation and migration

AU - Guedes, Marta

AU - Araújo, João R.

AU - Correia-Branco, Ana

AU - Gregório, Inês

AU - Martel, Fátima

AU - Keating, Elisa

N1 - Funding Information: This work was supported by Fundação para a Ciência e a Tecnologia (FCT), COMPETE, QREN and FEDER ( PTDC/SAU-OSM/102239/2008 ; SFRH/BPD/40170/2007 ) and by KLEAR Project (NORTE-07–0124-FEDER-000052), cofunded by FEDER and FCT. Publisher Copyright: © 2016 Elsevier Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2016/2/15

Y1 - 2016/2/15

N2 - This work aimed to characterize the uptake of folate and glucose by breast cancer cells and to study the effect of lactate upon the transport of these nutrients and upon cell viability, proliferation and migration capacity.Data obtained showed that: a) MCF7 cells uptake 3H-folic acid (3H-FA) at physiological but not at acidic pH; b) T47D cells accumulate 3H-FA and 14C-5-methyltetrahydrofolate (14C-5-MTHF) more efficiently at acidic than at physiological pH; c) 3H-deoxyglucose (3H-DG) uptake by T47D cells is sodium-independent, inhibited by cytochalasin B (CYT B) and stimulated by insulin.Regarding the effect of lactate, in T47D cells, acute (26 min) and chronic (24 h) exposure to lactic acid (LA) stimulated 3H-FA uptake. Acute exposure to LA also stimulated 3H-DG uptake and chronic exposure to LA significantly stimulated T47D cell migratory capacity.In conclusion, the transport of folates is strikingly different in two phenotypically similar breast cancer cell lines: MCF7 and T47D cells. Additionally, lactate seems to act as a signaling molecule which increases the uptake of nutrients and promotes the migration capacity of T47D cells.

AB - This work aimed to characterize the uptake of folate and glucose by breast cancer cells and to study the effect of lactate upon the transport of these nutrients and upon cell viability, proliferation and migration capacity.Data obtained showed that: a) MCF7 cells uptake 3H-folic acid (3H-FA) at physiological but not at acidic pH; b) T47D cells accumulate 3H-FA and 14C-5-methyltetrahydrofolate (14C-5-MTHF) more efficiently at acidic than at physiological pH; c) 3H-deoxyglucose (3H-DG) uptake by T47D cells is sodium-independent, inhibited by cytochalasin B (CYT B) and stimulated by insulin.Regarding the effect of lactate, in T47D cells, acute (26 min) and chronic (24 h) exposure to lactic acid (LA) stimulated 3H-FA uptake. Acute exposure to LA also stimulated 3H-DG uptake and chronic exposure to LA significantly stimulated T47D cell migratory capacity.In conclusion, the transport of folates is strikingly different in two phenotypically similar breast cancer cell lines: MCF7 and T47D cells. Additionally, lactate seems to act as a signaling molecule which increases the uptake of nutrients and promotes the migration capacity of T47D cells.

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KW - Folate uptake

KW - Glucose uptake

KW - Lactate

KW - Warburg effect

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DO - 10.1016/j.yexcr.2016.01.008

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SN - 0014-4827

VL - 341

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Modulation of the uptake of critical nutrients by breast cancer cells by lactate: impact on cell survival, proliferation and migration

Abstract

Keywords

UN SDGs

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