TY - JOUR
T1 - Multi-step subcritical water extracts of fucus vesiculosus l. And codium tomentosum stackhouse
T2 - composition, health-benefits and safety
AU - Soares, Cristina
AU - Paíga, Paula
AU - Marques, Marta
AU - Neto, Tânia
AU - Carvalho, Ana Paula
AU - Paiva, Alexandre
AU - Simões, Pedro
AU - Costa, Leonor
AU - Bernardo, Ana
AU - Fernández, Naiara
AU - Pinto, Edgar
AU - Almeida, Agostinho
AU - Bronze, Maria Rosário
AU - Delerue-Matos, Cristina
AU - Grosso, Clara
N1 - Funding Information:
Funding: This work received financial support from EU and Fundação para a Ciência e a Tecnolo-gia (FCT) through the project PTDC/OCE-ETA/30240/2017-SilverBrain-From sea to brain: Green neuroprotective extracts for nanoencapsulation and functional food production (PO-CI-01-0145-FEDER-030240).
Funding Information:
Acknowledgments: This work was supported by projects REQUIMTE/LAQV—UIDB/50006/2020 and UIDP/50006/2020, and iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020, financed by FCT/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), through national funds. AP also acknowledge FCT for the project IF/01146/2015. We acknowledge the help of Nuno Costa and Carla Rodrigues from Laboratório de Análises (LAQV, REQUIMTE).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/5
Y1 - 2021/5
N2 - Mental health and active aging are two of the main concerns in the 21st century. To search for new neuroprotective compounds, extracts of Codium tomentosum Stackhouse and Fucus vesiculosus L. were obtained through multi-step (four step) subcritical water extraction using a temperature gradient. The safety assessment of the extracts was performed by screening pharmaceutical compounds and pesticides by UHPLC-MS/MS, and iodine and arsenic levels by ICP-MS. Although the extracts were free of pharmaceutical compounds and pesticides, the presence of arsenic and high iodine contents were found in the first two extraction steps. Thus, the health-benefits were only evaluated for the fractions obtained in steps 3 and 4 from the extraction process. These fractions were tested against five brain enzymes implicated in Alzheimer’s, Parkinson’s, and major depression etiology as well as against reactive oxygen and nitrogen species, having been observed a strong enzyme inhibition and radical scavenging activities for the step 4 fractions from both seaweed species. Regarding the variation of the chemical composition during the extraction, step 1 fractions were the richest in phenolic compounds. With the increase in temperature, Maillard reaction, caramelization and thermo-oxidation occurred, and the resulting products positively affected the antioxidant capacity and the neuroprotective effects.
AB - Mental health and active aging are two of the main concerns in the 21st century. To search for new neuroprotective compounds, extracts of Codium tomentosum Stackhouse and Fucus vesiculosus L. were obtained through multi-step (four step) subcritical water extraction using a temperature gradient. The safety assessment of the extracts was performed by screening pharmaceutical compounds and pesticides by UHPLC-MS/MS, and iodine and arsenic levels by ICP-MS. Although the extracts were free of pharmaceutical compounds and pesticides, the presence of arsenic and high iodine contents were found in the first two extraction steps. Thus, the health-benefits were only evaluated for the fractions obtained in steps 3 and 4 from the extraction process. These fractions were tested against five brain enzymes implicated in Alzheimer’s, Parkinson’s, and major depression etiology as well as against reactive oxygen and nitrogen species, having been observed a strong enzyme inhibition and radical scavenging activities for the step 4 fractions from both seaweed species. Regarding the variation of the chemical composition during the extraction, step 1 fractions were the richest in phenolic compounds. With the increase in temperature, Maillard reaction, caramelization and thermo-oxidation occurred, and the resulting products positively affected the antioxidant capacity and the neuroprotective effects.
KW - Bioactivities
KW - Environmental contaminants
KW - Iodine
KW - Neuroprotection
KW - Reactive nitrogen species
KW - Reactive oxygen species
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=85106876665&partnerID=8YFLogxK
U2 - 10.3390/pr9050893
DO - 10.3390/pr9050893
M3 - Article
AN - SCOPUS:85106876665
SN - 2227-9717
VL - 9
JO - Processes
JF - Processes
IS - 5
M1 - 893
ER -
