Myonuclear position and blood vessel organization during skeletal muscle postnatal development

Catarina Sequeira, Lou Martha Wackerbarth, Andreia Pena, Mafalda Sá-Pereira, Cláudio A. Franco, Edgar R. Gomes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Skeletal muscle development is a complex process involving myoblast fusion to generate multinucleated fibers. Myonuclei first align in the center of the myotubes before migrating to the periphery of the myofiber. Blood vessels (BVs) are important contributors to the correct development of skeletal muscle, and myonuclei are found next to BVs in adult muscle. Here, we show that most myonuclear migration to the periphery occurs between embryonic day 17.5 and postnatal day 1 in mouse. Furthermore, myonuclear accretion after postnatal day 7 does not result in centrally nucleated myofibers as observed in the embryo. Instead, myonuclei remain at the periphery of the myofiber without moving to the center. Finally, we show that hypovascularization of skeletal muscle alters the interaction between myonuclei and BVs, suggesting that BVs may contribute to myonuclear positioning during skeletal muscle postnatal development. Overall, this work provides a comprehensive analysis of skeletal muscle development during the highly dynamic postnatal period, bringing new insights about myonuclear positioning and its interaction with BVs.

Original languageEnglish
Article numberdev202548
JournalDevelopment (Cambridge)
Volume151
Issue number19
DOIs
Publication statusPublished - Oct 2024

Keywords

  • Blood vessels
  • Mouse
  • Nuclear positioning
  • Postnatal development
  • Skeletal muscle

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