NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusitanian toadfish halobatrachus didactylus body mucus

Marta Fernandez Cunha; Ezequiel R. Coscueta; María Emilia Brassesco; Frederico Almada; David Gonçalves; Manuela Pintado

Abstract

The mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC revealed a prominent peak at 775 Dalton. The mucus samples were pooled, fractionated, and analyzed by nanoLC-MS/MS. Database identification was unreliable, so de novo sequencing with PEAKS Studio was used, resulting in five peptides selected for their predicted bioactivities.

Original language	English
Pages	1-1
Number of pages	1
Publication status	Published - 3 Jul 2024
Event	Encontro Ciência 2024 - Alfândega do Porto, Porto, Portugal Duration: 3 Jul 2024 → 5 Jul 2024

Conference

Conference	Encontro Ciência 2024
Country/Territory	Portugal
City	Porto
Period	3/07/24 → 5/07/24

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114060193Accepted author manuscript, 168 KBLicence: Unspecified

http://hdl.handle.net/10400.14/48103Licence: Unspecified

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1 Article

Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from in silico insights to essential in vitro validation
Cunha, M. F., Coscueta, E. R., Brassesco, M. E., Almada, F., Gonçalves, D. & Pintado, M. M., 13 Feb 2025, In: Marine Drugs. 23, 2, 25 p., 82.
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NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusitanian Toadfish Halobatrachus didactylus body mucus
Cunha, M. F., Coscueta, E. R., Brassesco, M. E., Almada, F., Gonçalves, D. & Pintado, M., 3 Jul 2024, p. 1-1. 1 p.
Research output: Contribution to conference › Poster › peer-review

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title = "NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusitanian toadfish halobatrachus didactylus body mucus",

abstract = "The mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC revealed a prominent peak at 775 Dalton. The mucus samples were pooled, fractionated, and analyzed by nanoLC-MS/MS. Database identification was unreliable, so de novo sequencing with PEAKS Studio was used, resulting in five peptides selected for their predicted bioactivities.",

author = "Cunha, \{Marta Fernandez\} and Coscueta, \{Ezequiel R.\} and Brassesco, \{Mar{\'i}a Emilia\} and Frederico Almada and David Gon{\c c}alves and Manuela Pintado",

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AU - Cunha, Marta Fernandez

AU - Coscueta, Ezequiel R.

AU - Brassesco, María Emilia

AU - Almada, Frederico

AU - Gonçalves, David

AU - Pintado, Manuela

PY - 2024/7/3

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N2 - The mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC revealed a prominent peak at 775 Dalton. The mucus samples were pooled, fractionated, and analyzed by nanoLC-MS/MS. Database identification was unreliable, so de novo sequencing with PEAKS Studio was used, resulting in five peptides selected for their predicted bioactivities.

AB - The mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC revealed a prominent peak at 775 Dalton. The mucus samples were pooled, fractionated, and analyzed by nanoLC-MS/MS. Database identification was unreliable, so de novo sequencing with PEAKS Studio was used, resulting in five peptides selected for their predicted bioactivities.

M3 - Abstract

SP - 1

EP - 1

T2 - Encontro Ciência 2024

Y2 - 3 July 2024 through 5 July 2024

ER -

NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusitanian toadfish halobatrachus didactylus body mucus

Abstract

Conference

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Research output

Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from in silico insights to essential in vitro validation

NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusitanian Toadfish Halobatrachus didactylus body mucus

Cite this