TY - JOUR
T1 - Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis
AU - Oliveira, Diana
AU - Wilbey, Andrew
AU - Grandison, Alistair S.
AU - Roseiro, Luisa Bivar
PY - 2014/3
Y1 - 2014/3
N2 - The functional food market is growing rapidly and membrane processing offers several advantages over conventional methods for separation, fractionation and recovery of bioactive components. The aim of the present study was to select a process that could be implemented easily on an industrial scale for the isolation of natural lactose-derived oligosaccharides (OS) from caprine whey, enabling the development of functional foods for clinical and infant nutrition. The most efficient process was the combination of a pre-treatment to eliminate proteins and fat, using an ultrafiltration (UF) membrane of 25-kDa molecular weight cutoff (MWCO), followed by a tighter UF membrane with 1-kDa MWCO. Circa 90 % of the carbohydrates recovered in the final retentate were OS. Capillary electrophoresis was used to evaluate the OS profile in this retentate. The combined membrane-processing system is thus a promising technique for obtaining natural concentrated OS from whey.
AB - The functional food market is growing rapidly and membrane processing offers several advantages over conventional methods for separation, fractionation and recovery of bioactive components. The aim of the present study was to select a process that could be implemented easily on an industrial scale for the isolation of natural lactose-derived oligosaccharides (OS) from caprine whey, enabling the development of functional foods for clinical and infant nutrition. The most efficient process was the combination of a pre-treatment to eliminate proteins and fat, using an ultrafiltration (UF) membrane of 25-kDa molecular weight cutoff (MWCO), followed by a tighter UF membrane with 1-kDa MWCO. Circa 90 % of the carbohydrates recovered in the final retentate were OS. Capillary electrophoresis was used to evaluate the OS profile in this retentate. The combined membrane-processing system is thus a promising technique for obtaining natural concentrated OS from whey.
KW - Capillary electrophoresis profile
KW - Caprine milk whey
KW - Membrane separation
KW - Oligosaccharides
KW - Ultrafiltration
UR - http://www.scopus.com/inward/record.url?scp=84895060073&partnerID=8YFLogxK
U2 - 10.1007/s11947-013-1153-1
DO - 10.1007/s11947-013-1153-1
M3 - Article
SN - 1935-5130
VL - 7
SP - 915
EP - 920
JO - Food and Bioprocess Technology
JF - Food and Bioprocess Technology
IS - 3
ER -