TY - JOUR
T1 - New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
AU - Ribeiro, Nilza
AU - Sousa, Aureliana
AU - Cunha-Reis, Cassilda
AU - Oliveira, Ana Leite
AU - Granja, Pedro L.
AU - Monteiro, Fernando J.
AU - Sousa, Susana R.
N1 - Funding Information:
The authors would like to acknowledge the support from Fundação para a Ciência e a Tecnologia, Portugal, for N. Ribeiro's PhD grant (SFRH/BD/69686/2010) and A. Sousa position (UID/BIM/04293/2013/POCI-01-0145-FEDER-007274). Also, the provision of nanoHA (nanoXIM) by FLUIDINOVA, S.A. (Portugal) and collagen by Kensey Nash (USA) is greatly acknowledged. The authors are thankful to Maria Pia Ferraz (UFP) for her support with the microbiology facilities. The authors acknowledge the support of the i3S Scientific Platforms “Bioimaging” and “BioSciences Screening”, members of the national infrastructure Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122).
Funding Information:
The authors would like to acknowledge the support from Fundação para a Ciência e a Tecnologia, Portugal, for N. Ribeiro's PhD grant (SFRH/BD/69686/2010) and A. Sousa position (UID/BIM/04293/2013/POCI-01-0145-FEDER-007274). Also, the provision of nanoHA (nanoXIM) by FLUIDINOVA, S.A. (Portugal) and collagen by Kensey Nash (USA) is greatly acknowledged. The authors are thankful to Maria Pia Ferraz (UFP) for her support with the microbiology facilities. The authors acknowledge the support of the i3S Scientific Platforms “Bioimaging” and “BioSciences Screening”, members of the national infrastructure Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122). Nilza Ribeiro, Aureliana Sousa, Cassilda Cunha-Reis, Ana Leite Oliveira, Pedro L. Granja, Fernando J. Monteiro, Susana R. Sousa contributed to the study design, conceptualization and methodology. Nilza Ribeiro and Aureliana Sousa conducted the research and investigation process performing the experimental studies and both drew the schemes and figures, contributing equally to the manuscript. Cassilda Cunha-Reis and Ana Leite Oliveira were responsible for the mechanical tests and all authors analyzed the data and approved the final manuscript written by Nilza Ribeiro and Aureliana Sousa.
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/4
Y1 - 2021/4
N2 - This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.
AB - This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.
KW - Calcium ions
KW - Collagen electrospinning
KW - Composite biomaterial
KW - Hydroxyapatite electrospraying
KW - Skin wound healing
UR - http://www.scopus.com/inward/record.url?scp=85100203422&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2020.102353
DO - 10.1016/j.nano.2020.102353
M3 - Article
C2 - 33421622
AN - SCOPUS:85100203422
SN - 1549-9634
VL - 33
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
M1 - 102353
ER -