Non-ionic aqueous micellar extraction of trypsin inhibitors and isoflavones from soybean meal: process optimization

Ezequiel R. Coscueta*, María Emilia Brassesco, Luciana Pellegrini Malpiedi, Bibiana Nerli

*Corresponding author for this work

Research output: Working paperPreprint



Simultaneous extraction of trypsin inhibitors and soy isoflavones from soybean meal was investigated using the non-destructive phytochemical extraction process, namely aqueous micellar system. The ethoxylated aliphatic alcohols Genapol X-080, Tergitol 15-S-7, and Tergitol 15-S-9, all non-toxic and biodegradable surfactants, were assessed as potential extractants. A Box-Behnken multifactorial design with the application of the Derringer desirability was used to determine the conditions that maximized the trypsin inhibitors and isoflavone extraction while minimizing the protein extraction. The optimum condition of 5% m/m of surfactant in 50 mM aqueous sodium citrate solution pH 4.5, at 45 °C for 45 min, was established for the three surfactants. The novel methodology would allow the extraction of the main soybean antinutritional factors, trypsin inhibitors, and the valuable isoflavones, preserving the nutritional quality of the treated material. This represents a sustainable alternative methodology for industrial purposes due to its low cost, biodegradability, non-toxicity, and easy scaling up.
Original languageEnglish
PublisherMDPI Multidisciplinary Digital Publishing Institute
Number of pages14
Publication statusPublished - 17 Jan 2022


  • Aqueous micellar systems
  • Isoflavone extraction
  • Multifactorial optimization
  • Non-ionic surfactant
  • Trypsin inhibitor


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