TY - JOUR
T1 - Noninvasive imaging and quantification of bile salt hydrolase activity
T2 - from bacteria to humans
AU - Khodakivskyi, Pavlo V.
AU - Lauber, Christian L.
AU - Yevtodiyenko, Aleksey
AU - Bazhin, Arkadiy A.
AU - Bruce, Stephen
AU - Ringel-Kulka, Tamar
AU - Ringel, Yehuda
AU - Bétrisey, Bertrand
AU - Torres, Joana
AU - Hu, Jianzhong
AU - Chou, Chieh Jason
AU - Goun, Elena A.
N1 - Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2021/2/3
Y1 - 2021/2/3
N2 - The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gastrointestinal tract of mice and humans. Using this assay, we showed that certain types of prebiotics are capable of increasing BSH activity of the gut microbiota in vivo and successfully demonstrated potential application of this assay as a noninvasive diagnostic test to predict the clinical status of inflammatory bowel disease (IBD) patients.
AB - The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gastrointestinal tract of mice and humans. Using this assay, we showed that certain types of prebiotics are capable of increasing BSH activity of the gut microbiota in vivo and successfully demonstrated potential application of this assay as a noninvasive diagnostic test to predict the clinical status of inflammatory bowel disease (IBD) patients.
UR - http://www.scopus.com/inward/record.url?scp=85101024312&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aaz9857
DO - 10.1126/sciadv.aaz9857
M3 - Article
C2 - 33536224
AN - SCOPUS:85101024312
SN - 2375-2548
VL - 7
JO - Science advances
JF - Science advances
IS - 6
M1 - eaaz9857
ER -