TY - JOUR
T1 - Novel linear polymers able to inhibit bacterial quorum sensing
AU - Cavaleiro, Eliana
AU - Duarte, Ana Sofia
AU - Esteves, Ana Cristina
AU - Correia, Antõnio
AU - Whitcombe, Michael J.
AU - Piletska, Elena V.
AU - Piletsky, Sergey A.
AU - Chianella, Iva
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Bacterial phenotypes, such as biofilm formation, antibiotic resistance and virulence expression, are associated with quorum sensing. Quorum sensing is a density-dependent regulatory system of gene expression controlled by specific signal molecules, such as N-acyl homoserine lactones (AHLs), produced and released by bacteria. This study reports the development of linear polymers capable to attenuate quorum sensing by adsorption of AHLs. Linear polymers were synthesized using MMA as backbone monomer and methacrylic acid and itaconic acid as functional monomers. Two different quorum sensing-controlled phenotypes, Vibrio fischeri bioluminescence and Aeromonas hydrophila biofilm formation, were evaluated to test the polymers' efficiency. Results showed that both phenotypes were significantly affected by the polymers, with the itaconic acid-containing material being more effective than the methacrylic acid one. The polymer inhibitory effects were reverted by the addition of lactones, confirming attenuation of quorum sensing through sequestration of signal molecules. The polymers also showed no cytotoxicity when tested using a mammalian cell line.
AB - Bacterial phenotypes, such as biofilm formation, antibiotic resistance and virulence expression, are associated with quorum sensing. Quorum sensing is a density-dependent regulatory system of gene expression controlled by specific signal molecules, such as N-acyl homoserine lactones (AHLs), produced and released by bacteria. This study reports the development of linear polymers capable to attenuate quorum sensing by adsorption of AHLs. Linear polymers were synthesized using MMA as backbone monomer and methacrylic acid and itaconic acid as functional monomers. Two different quorum sensing-controlled phenotypes, Vibrio fischeri bioluminescence and Aeromonas hydrophila biofilm formation, were evaluated to test the polymers' efficiency. Results showed that both phenotypes were significantly affected by the polymers, with the itaconic acid-containing material being more effective than the methacrylic acid one. The polymer inhibitory effects were reverted by the addition of lactones, confirming attenuation of quorum sensing through sequestration of signal molecules. The polymers also showed no cytotoxicity when tested using a mammalian cell line.
KW - Aeromonas hydrophila
KW - Copolymers
KW - Quorum sensing
KW - Vibrio fischeri
UR - http://www.scopus.com/inward/record.url?scp=84929082424&partnerID=8YFLogxK
U2 - 10.1002/mabi.201400447
DO - 10.1002/mabi.201400447
M3 - Article
C2 - 25626858
AN - SCOPUS:84929082424
SN - 1616-5187
VL - 15
SP - 647
EP - 656
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 5
ER -