NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells

Ana Rita Álvaro; Joana Rosmaninho-Salgado; Ana Raquel Santiago; João Martins; Célia Aveleira; Paulo F. Santos; Tiago Pereira; Denisa Gouveia; Ana Luísa Carvalho; Eric Grouzmann; António Francisco Ambrósio; Cláudia Cavadas

doi:10.1016/j.neuint.2007.01.010

NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells

Ana Rita Álvaro, Joana Rosmaninho-Salgado, Ana Raquel Santiago, João Martins, Célia Aveleira, Paulo F. Santos, Tiago Pereira, Denisa Gouveia, Ana Luísa Carvalho, Eric Grouzmann, António Francisco Ambrósio, Cláudia Cavadas^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Müller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y1, Y2, Y4 and Y5 receptors. Retina endothelial cells express all NPY receptors except NPY Y5 receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Müller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.

Original language	English
Pages (from-to)	757-763
Number of pages	7
Journal	Neurochemistry International
Volume	50
Issue number	5
DOIs	https://doi.org/10.1016/j.neuint.2007.01.010
Publication status	Published - Apr 2007
Externally published	Yes

Keywords

Endothelial cells
Microglial cells
Müller cells
Neuropeptide Y (NPY)
NPY receptors
NPY release
Rat retina

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10.1016/j.neuint.2007.01.010

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Álvaro, A. R., Rosmaninho-Salgado, J., Santiago, A. R., Martins, J., Aveleira, C., Santos, P. F., Pereira, T., Gouveia, D., Carvalho, A. L., Grouzmann, E., Ambrósio, A. F., & Cavadas, C. (2007). NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells. Neurochemistry International, 50(5), 757-763. https://doi.org/10.1016/j.neuint.2007.01.010

@article{be7640dc645a4bd4b384690b89021a9f,

title = "NPY in rat retina is present in neurons, in endothelial cells and also in microglial and M{\"u}ller cells",

abstract = "NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat M{\"u}ller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y1, Y2, Y4 and Y5 receptors. Retina endothelial cells express all NPY receptors except NPY Y5 receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat M{\"u}ller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.",

keywords = "Endothelial cells, Microglial cells, M{\"u}ller cells, Neuropeptide Y (NPY), NPY receptors, NPY release, Rat retina",

author = "{\'A}lvaro, {Ana Rita} and Joana Rosmaninho-Salgado and Santiago, {Ana Raquel} and Jo{\~a}o Martins and C{\'e}lia Aveleira and Santos, {Paulo F.} and Tiago Pereira and Denisa Gouveia and Carvalho, {Ana Lu{\'i}sa} and Eric Grouzmann and Ambr{\'o}sio, {Ant{\'o}nio Francisco} and Cl{\'a}udia Cavadas",

note = "Funding Information: This work was supported by Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, Portugal (POCTI/CBO/38545/01, SFRH/BD/10394/2002, SFRH/BD9690/2002, SFRH/BD/12900/2003, SFRH/BD/18827/2004) and FEDER. The endothelial and the M{\"u}ller cell lines were kindly given by Dr. Ken-ichi Hosoya (Faculty of Pharmacological Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2007",

month = apr,

doi = "10.1016/j.neuint.2007.01.010",

language = "English",

volume = "50",

pages = "757--763",

journal = "Neurochemistry International",

issn = "0197-0186",

publisher = "Elsevier Ltd.",

number = "5",

}

Álvaro, AR, Rosmaninho-Salgado, J, Santiago, AR, Martins, J, Aveleira, C, Santos, PF, Pereira, T, Gouveia, D, Carvalho, AL, Grouzmann, E, Ambrósio, AF & Cavadas, C 2007, 'NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells', Neurochemistry International, vol. 50, no. 5, pp. 757-763. https://doi.org/10.1016/j.neuint.2007.01.010

TY - JOUR

T1 - NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells

AU - Álvaro, Ana Rita

AU - Rosmaninho-Salgado, Joana

AU - Santiago, Ana Raquel

AU - Martins, João

AU - Aveleira, Célia

AU - Santos, Paulo F.

AU - Pereira, Tiago

AU - Gouveia, Denisa

AU - Carvalho, Ana Luísa

AU - Grouzmann, Eric

AU - Ambrósio, António Francisco

AU - Cavadas, Cláudia

N1 - Funding Information: This work was supported by Fundação para a Ciência e a Tecnologia, Portugal (POCTI/CBO/38545/01, SFRH/BD/10394/2002, SFRH/BD9690/2002, SFRH/BD/12900/2003, SFRH/BD/18827/2004) and FEDER. The endothelial and the Müller cell lines were kindly given by Dr. Ken-ichi Hosoya (Faculty of Pharmacological Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/4

Y1 - 2007/4

N2 - NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Müller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y1, Y2, Y4 and Y5 receptors. Retina endothelial cells express all NPY receptors except NPY Y5 receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Müller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.

AB - NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Müller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y1, Y2, Y4 and Y5 receptors. Retina endothelial cells express all NPY receptors except NPY Y5 receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Müller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.

KW - Endothelial cells

KW - Microglial cells

KW - Müller cells

KW - Neuropeptide Y (NPY)

KW - NPY receptors

KW - NPY release

KW - Rat retina

UR - http://www.scopus.com/inward/record.url?scp=33947389655&partnerID=8YFLogxK

U2 - 10.1016/j.neuint.2007.01.010

DO - 10.1016/j.neuint.2007.01.010

M3 - Article

C2 - 17353067

AN - SCOPUS:33947389655

SN - 0197-0186

VL - 50

SP - 757

EP - 763

JO - Neurochemistry International

JF - Neurochemistry International

IS - 5

ER -

NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells

Abstract

Keywords

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