Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

Lara R.P. Amorim, Juan A.C. Tamames, Joana G.L. Silva, Paul A. Gibbs, Paula C. Teixeira*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2α, 135KP and 12′ phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.

Original languageEnglish
Pages (from-to)754-759
Number of pages6
JournalFood Control
Volume21
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • Bacteriophages
  • Cluster analysis
  • Discriminant analysis
  • Lytic spectra
  • Phage typing
  • Salmonella spp.
  • Spot test

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