Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

Lara R.P. Amorim; Juan A.C. Tamames; Joana G.L. Silva; Paul A. Gibbs; Paula C. Teixeira

doi:10.1016/j.foodcont.2009.11.004

Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

Lara R.P. Amorim
, Juan A.C. Tamames
, Joana G.L. Silva
, Paul A. Gibbs
, Paula C. Teixeira^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2α, 135KP and 12′ phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.

Original language	English
Pages (from-to)	754-759
Number of pages	6
Journal	Food Control
Volume	21
Issue number	5
DOIs	https://doi.org/10.1016/j.foodcont.2009.11.004
Publication status	Published - May 2010

Keywords

Bacteriophages
Cluster analysis
Discriminant analysis
Lytic spectra
Phage typing
Salmonella spp.
Spot test

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10.1016/j.foodcont.2009.11.004

19793062Accepted author manuscript, 550 KBLicence: CC BY-NC-ND

http://hdl.handle.net/10400.14/4850Licence: CC BY-NC-ND

Cite this

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title = "Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages",

abstract = "This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2α, 135KP and 12′ phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.",

keywords = "Bacteriophages, Cluster analysis, Discriminant analysis, Lytic spectra, Phage typing, Salmonella spp., Spot test",

author = "Amorim, \{Lara R.P.\} and Tamames, \{Juan A.C.\} and Silva, \{Joana G.L.\} and Gibbs, \{Paul A.\} and Teixeira, \{Paula C.\}",

year = "2010",

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doi = "10.1016/j.foodcont.2009.11.004",

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journal = "Food Control",

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T1 - Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

AU - Amorim, Lara R.P.

AU - Tamames, Juan A.C.

AU - Silva, Joana G.L.

AU - Gibbs, Paul A.

AU - Teixeira, Paula C.

PY - 2010/5

Y1 - 2010/5

N2 - This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2α, 135KP and 12′ phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.

AB - This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2α, 135KP and 12′ phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.

KW - Bacteriophages

KW - Cluster analysis

KW - Discriminant analysis

KW - Lytic spectra

KW - Phage typing

KW - Salmonella spp.

KW - Spot test

UR - https://www.scopus.com/pages/publications/73549097502

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DO - 10.1016/j.foodcont.2009.11.004

M3 - Article

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VL - 21

SP - 754

EP - 759

JO - Food Control

JF - Food Control

IS - 5

ER -

Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

Abstract

Keywords

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