@article{c6972160ad534705922d611946dc4db1,
title = "Occurrence of Salmonella spp. in eggs from backyard chicken flocks in Portugal and Romania - results of a preliminary study",
abstract = "The aim of this study was to conduct a preliminary investigation on the occurrence of Salmonella spp. in eggs from chickens raised in backyards in Portugal and Romania. A lack of compliance with safety practices by chicken owners, was demonstrated, especially in Portugal, as 96% of the eggs were visibly dirty and 92.5% were stored at room temperature. In Romania the 202 analysed eggs were Salmonella free, whereas in Portugal six of the 200 eggs sampled were positive for Salmonella spp. (3%). A positive egg for Salmonella spp. was found in 10.7% of the 56 backyard flocks sampled in Portugal. One egg exhibited contamination both in the shell-membrane mixture and in its content, while in the remaining eggs, the pathogen was found either in the shell-membrane (n = 2) or in the yolk and white mixture (n = 3). The serotypes S. Typhimurium (with identical PFGE patterns) and S. Enteritidis were isolated from five eggs and one egg, respectively. Whilst S. Enteritidis was sensitive to the 14 antibiotics tested, S. Typhimurium isolates presented divergent antimicrobial resistant phenotypes and three were classified as multi-drug resistant.",
keywords = "Consumer preferences, Henhouse, Multi-drug resistance (MDR), S. enterica, S. Typhimurium, Storage",
author = "V. Ferreira and Cardoso, {M. J.} and R. Magalh{\~a}es and R. Maia and C. Neagu and L. Dumitra{\c s}cu and Nicolau, {A. I.} and P. Teixeira",
note = "Funding Information: This work was supported by SafeConsume –European Union Horizon 2020 Grant Agreement No 727580. We would also like to thank the scientific collaboration under the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT) project UID/Multi/50016/2019. We wish to thank M{\'o}nica Oleastro and Leonor Silveira from the Portuguese National Reference Laboratory Instituto Nacional de Sa{\'u}de Doutor Ricardo Jorge for confirming the Salmonella serotypes. SafeConsume participants in Work Package 3, led by Joachim Scholderer (University of Zurich, Switzerland) are acknowledged for organizing the consumer survey and collecting data (http://safeconsume.eu/about/timeline); scientific discussions with Solveig Langsrud (Project coordinator; Nofima, Norway) were relevant in the preparation of the manuscript. We thank Dr Paul Gibbs for his willingness to edit this manuscript. Funding Information: This work was supported by SafeConsume – European Union Horizon 2020 Grant Agreement No 727580 . We would also like to thank the scientific collaboration under the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT) project UID/Multi/50016/2019 . Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2020",
month = jul,
doi = "10.1016/j.foodcont.2020.107180",
language = "English",
volume = "113",
pages = "1--6",
journal = "Food Control",
issn = "0956-7135",
publisher = "Elsevier B.V.",
}