Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system

Adriana M. Pimenta*, M. Renata S. Souto, Rita I. L. Catarino, M. Fernanda C. Leal, José L. F. Costa Lima

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
1 Downloads

Abstract

An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 × 10 -5 M, a linear response over the concentrations of 2 × 10 -5 to 5 × 10 -3 M (slope of 57.4 mV decade -1 ) and a wide working pH range (2.1-6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.
Original languageEnglish
Pages (from-to)893-898
Number of pages6
JournalAnalytical Sciences
Volume29
Issue number9
DOIs
Publication statusPublished - 2013

Keywords

  • Automatic determination
  • Ofloxacin
  • Pharmaceuticals
  • Serum
  • Urine

Fingerprint

Dive into the research topics of 'Ofloxacin determination in urine, serum and pharmaceuticals using an automatic flow potentiometric system'. Together they form a unique fingerprint.

Cite this