TY - JOUR
T1 - Optimization of antioxidant activity and bioactive compounds extraction of winter savory leaves by high hydrostatic pressure
AU - Moreira, Sílvia A.
AU - Pintado, Manuela E.
AU - Saraiva, Jorge A.
N1 - Funding Information:
Silvia A. Moreira is grateful for the financial support of this work from FCT through the Doctoral Grant SFRH/BD/110430/2015.
Funding Information:
Thanks are due to the University of Aveiro and Funda??o para a Ci?ncia e Tecnologia/Minist?rio da Ci?ncia e Tecnologia for the financial support for the QOPNA & LAQV-REQUIMTE research unit (FCT UID/QUI/00062/2019 and UIDB/50006/2020) and CBQF (FCT UID/Multi/50016/2019) through national funds and, where applicable, co-financed by the Fundo Europeu de Desenvolvimento Regional (FEDER), within the PT2020 Partnership Agreement. Silvia A. Moreira is grateful for the financial support of this work from FCT through the Doctoral Grant SFRH/BD/110430/2015.
Publisher Copyright:
© 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Winter savory is often used in traditional medicine, having several recognized biological properties. This study aimed to evaluate the effect of high pressure-assisted extraction (HPE) and its optimization by response surface methodology to obtain winter savory extracts with a high content of bioactive compounds and high antioxidant activity (the effect of extraction pressure, extraction time, and ethanol concentration were investigated). Results showed that data could be well fitted to second-order polynomial mathematical models for total phenolics, total flavonoids, chlorophylls, carotenoids, and extraction yield, as well as for antioxidant activity. The models predicted optimal conditions ranging 200–500 MPa, extraction time 1–20 min, and ethanol concentration 0–70% (v,v). In comparison with extraction at atmospheric pressure, HPE showed to be more efficient, allowing obtaining an increase of about 40% for all compounds, and an increase of 29, 48, and 70% for antioxidant activity by FRAP, DPPH and ABTS assays, respectively.
AB - Winter savory is often used in traditional medicine, having several recognized biological properties. This study aimed to evaluate the effect of high pressure-assisted extraction (HPE) and its optimization by response surface methodology to obtain winter savory extracts with a high content of bioactive compounds and high antioxidant activity (the effect of extraction pressure, extraction time, and ethanol concentration were investigated). Results showed that data could be well fitted to second-order polynomial mathematical models for total phenolics, total flavonoids, chlorophylls, carotenoids, and extraction yield, as well as for antioxidant activity. The models predicted optimal conditions ranging 200–500 MPa, extraction time 1–20 min, and ethanol concentration 0–70% (v,v). In comparison with extraction at atmospheric pressure, HPE showed to be more efficient, allowing obtaining an increase of about 40% for all compounds, and an increase of 29, 48, and 70% for antioxidant activity by FRAP, DPPH and ABTS assays, respectively.
KW - Antioxidant activity
KW - Bioactive compounds
KW - High pressure extraction
KW - Response surface methodology
KW - Winter savory
UR - http://www.scopus.com/inward/record.url?scp=85094127110&partnerID=8YFLogxK
U2 - 10.1080/08957959.2020.1830079
DO - 10.1080/08957959.2020.1830079
M3 - Article
AN - SCOPUS:85094127110
SN - 0895-7959
VL - 40
SP - 543
EP - 560
JO - High Pressure Research
JF - High Pressure Research
IS - 4
ER -