Optimization of antioxidant activity and bioactive compounds extraction of winter savory leaves by high hydrostatic pressure

Sílvia A. Moreira, Manuela E. Pintado, Jorge A. Saraiva*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Winter savory is often used in traditional medicine, having several recognized biological properties. This study aimed to evaluate the effect of high pressure-assisted extraction (HPE) and its optimization by response surface methodology to obtain winter savory extracts with a high content of bioactive compounds and high antioxidant activity (the effect of extraction pressure, extraction time, and ethanol concentration were investigated). Results showed that data could be well fitted to second-order polynomial mathematical models for total phenolics, total flavonoids, chlorophylls, carotenoids, and extraction yield, as well as for antioxidant activity. The models predicted optimal conditions ranging 200–500 MPa, extraction time 1–20 min, and ethanol concentration 0–70% (v,v). In comparison with extraction at atmospheric pressure, HPE showed to be more efficient, allowing obtaining an increase of about 40% for all compounds, and an increase of 29, 48, and 70% for antioxidant activity by FRAP, DPPH and ABTS assays, respectively.

Original languageEnglish
Pages (from-to)543-560
Number of pages18
JournalHigh Pressure Research
Volume40
Issue number4
DOIs
Publication statusPublished - 1 Oct 2020

Keywords

  • Antioxidant activity
  • Bioactive compounds
  • High pressure extraction
  • Response surface methodology
  • Winter savory

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