Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

Débora A. Campos; Ezequiel R. Coscueta; Nadia Woitovich Valetti; Lorenzo M. Pastrana-Castro; José A. Teixeira; Guillermo A. Picó; Maria Manuela Pintado

doi:10.1016/j.foodhyd.2018.09.009

Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

Débora A. Campos, Ezequiel R. Coscueta, Nadia Woitovich Valetti, Lorenzo M. Pastrana-Castro, José A. Teixeira, Guillermo A. Picó, Maria Manuela Pintado^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).

Original language	English
Pages (from-to)	792-804
Number of pages	13
Journal	Food Hydrocolloids
Volume	87
DOIs	https://doi.org/10.1016/j.foodhyd.2018.09.009
Publication status	Published - Feb 2019

Keywords

Bromelain
Carrageenan
Natural crude juices
Protein complex

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10.1016/j.foodhyd.2018.09.009

http://hdl.handle.net/10400.14/25781

Cite this

@article{2df23b9023074f42adec11027e2d5905,

title = "Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation",

abstract = "A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).",

keywords = "Bromelain, Carrageenan, Natural crude juices, Protein complex",

author = "Campos, {D{\'e}bora A.} and Coscueta, {Ezequiel R.} and Valetti, {Nadia Woitovich} and Pastrana-Castro, {Lorenzo M.} and Teixeira, {Jos{\'e} A.} and Pic{\'o}, {Guillermo A.} and Pintado, {Maria Manuela}",

year = "2019",

month = feb,

doi = "10.1016/j.foodhyd.2018.09.009",

language = "English",

volume = "87",

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journal = "Food Hydrocolloids",

issn = "0268-005X",

publisher = "Elsevier Science",

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T1 - Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

AU - Campos, Débora A.

AU - Coscueta, Ezequiel R.

AU - Valetti, Nadia Woitovich

AU - Pastrana-Castro, Lorenzo M.

AU - Teixeira, José A.

AU - Picó, Guillermo A.

AU - Pintado, Maria Manuela

PY - 2019/2

Y1 - 2019/2

N2 - A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).

AB - A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).

KW - Bromelain

KW - Carrageenan

KW - Natural crude juices

KW - Protein complex

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DO - 10.1016/j.foodhyd.2018.09.009

M3 - Article

AN - SCOPUS:85053405030

SN - 0268-005X

VL - 87

SP - 792

EP - 804

JO - Food Hydrocolloids

JF - Food Hydrocolloids

ER -

Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

Abstract

Keywords

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BiVALBI: Biotechnologies to valorise the regional food biodiversity in Latin America

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Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

BiVALBI: Biotechnologies to valorise the regional food biodiversity in Latin America

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