Optimization of culture conditions for Gardnerella vaginalis biofilm formation

Daniela Machado, Ana Palmeira-de-Oliveira, Nuno Cerca*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Bacterial vaginosis is the leading vaginal disorder in women in reproductive age. Although bacterial vaginosis is related with presence of a biofilm composed predominantly by Gardnerella vaginalis, there has not been a detailed information addressing the environmental conditions that influence the biofilm formation of this bacterial species. Here, we evaluated the influence of some common culture conditions on G. vaginalis biofilm formation, namely inoculumconcentration, incubation period, feeding conditions and culture mediumcomposition. Our results showed that culture conditions strongly influenced G. vaginalis biofilm formation and that biofilm formation was enhanced when starting the culture with a higher inoculum, using a fed-batch system and supplementing the growth medium with maltose.
Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalJournal of Microbiological Methods
Publication statusPublished - 1 Nov 2015
Externally publishedYes


  • Bacterial vaginosis
  • Biofilms
  • Clinical isolates
  • Gardnerella vaginalis


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