Oryzisolibacter propanilivorax gen. Nov., sp. nov., a propanildegrading bacterium

Ivone Vaz-Moreira, Carlos Narciso-da-rocha, Ana Rita Lopes, Gilda Carvalho, Alexandre Lobo-da-Cunha, William B. Whitman, Cindy Snauwaert, Peter Vandamme, Célia M. Manaia, Olga C. Nunes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Strain EPL6T, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6T was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601T (96.8 %) and Melaminivora alkalimesophila CY1T (96.8 %). Strain EPL6T was able to grow in a temperature range of 15-37 °C, pH 6-9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q 8. The genomic DNA had a G+C content of 69.4±0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C16: 0, C18: 1ω7c and summed feature 3 (C16: 1ω7c/iso-C15: 02-OH). Comparison of the genome sequence of strain EPL6T and of its closest neighbours, Melaminivora alkalimesophila CY1T and Alicycliphilus denitrificans K601T, yielded values of ANI ≤84.1 % and of AAI ≤80.3 %. Therefore, the genetic, phylogenetic, phenotypic and chemotaxonomic characteristics support the classification of this organism into a new taxon. Considering the genetic divergence of strain EPL6T from the type strains of the closest species, which belong to distinct genera, we propose a new genus within the family Comamonadaceae, named Oryzisolibacter propanilivorax gen. nov., sp. nov., represented by the isolate EPL6T as the type strain of the species (=LMG 28427T=CECT 8927T).
Original languageEnglish
Article number002184
Pages (from-to)3752-3758
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Comamonadaceae
  • Herbicide degrader
  • New genus
  • Rice field soil

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