Oxygen diffusion in lupin nodules: I.visualization of diffusion barrier operation

P. P.M. Iannetta, C. De Lorenzo, E. K. James, M. Fernandez-pascual, J. I. Sprent, M. M. Lucas, J. F. Witty, M. R. De Felipe, F. R. Minchin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


Root nodules of Lupinus albus (L.) cv. Multolupa were subjected to short- and medium-term stresses by lowering rhizosphere temperature from 25 to 16°C (2 h), detopping plants (3 h), darkening plants (21 h) or exposing roots to 20 mol m-3 KNO3 for 4 d. All experimental treatments produced increases in oxygen diffusion resistance, compared with control plants. These correlated with structural changes in the nodule cortex, which is described in detail for the first time. The most noticeable change is the occlusion of intercellular spaces by a glycoprotein which was identified using the monoclonal antibody MAC236. This glycoprotein was also found surrounding bacteria in intercellular spaces of the cortex of control nodules.

Original languageEnglish
Pages (from-to)1461-1467
Number of pages7
JournalJournal of Experimental Botany
Issue number9
Publication statusPublished - Sept 1993
Externally publishedYes


  • Glycoprotein
  • Lupinus albus
  • Nitrogen fixation
  • Nodules
  • Oxygen diffusion resistance


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