Activities per year
Abstract
The mucus covers the fish's body, working as a protective barrier. Besides physical protection, mucus provides molecules that protect the fish from pathogens damaging 1,2. These include antimicrobial peptides secreted in the mucus, which play an essential role in defense against microbial pathogens since these belong to the innate immune system2,3. In this study, two adult Halobatrachus didactylus individuals were captured from the wild in Sesimbra. Then, mucus collection was performed by scraping the dorsal-lateral body of the fish with a sponge. Our objective was the identification of new peptides with bioactive potential in mucus samples by chromatography analysis. Size exclusion highperformance liquid chromatography (SE-HPLC) analysis performed on mucus samples from the two individuals revealed a similar profile with an intense highlight peak which resulted in a distribution of about 775 Dalton. With interest in that peak, the two mucus samples were pooled for fractionation by SEC. The resulting fraction was analyzed by liquid chromatography-tandem mass spectrometry (LCMS/MS) to identify the most probable peptide sequences. Identification from databases did not provide reliable results, indicating a lack of information on the matrix analyzed. We resorted to de novo sequencing with good results using PEAKS Studio software. Five identified peptides were selected according to their bioactivities predicted in silico. Furthermore, the five identified peptides were synthesized, and the molecular size was validated by SE-HPLC analysis. Overall, this chromatographic approach enabled the identification of promising peptides, which bioactivities will be evaluated in vitro in future work.
Original language | English |
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Title of host publication | Livro de resumos do 12º Encontro Nacional de Cromatografia e XIV WARPA |
Editors | Sílvia M. Rocha |
Place of Publication | Lisboa |
Publisher | Sociedade Portuguesa de Química |
Pages | 208-208 |
Number of pages | 1 |
ISBN (Electronic) | 9789898124371 |
Publication status | Published - 6 Dec 2022 |
Event | 12º Encontro Nacional de Cromatografia - Universidade de Aveiro, Aveiro, Portugal Duration: 6 Dec 2022 → 8 Dec 2022 https://12enc.events.chemistry.pt/ |
Conference
Conference | 12º Encontro Nacional de Cromatografia |
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Abbreviated title | 12ENC |
Country/Territory | Portugal |
City | Aveiro |
Period | 6/12/22 → 8/12/22 |
Internet address |
Keywords
- Fish
- Mucus
- Halobatrachus didactylus
- SE-HPLC
- LC-MS/MS
- Peptides
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Dive into the research topics of 'P82 Peptide fraction identification by SE-HPLC and LC-MS/MS analysis of the body mucus from Portugal coastal fish Halobatrachus didactylus'. Together they form a unique fingerprint.Activities
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12º Encontro Nacional de Cromatografia
Cunha, M. F. D. (Participant)
6 Dec 2022 → 8 Dec 2022Activity: Participating in or organising an event › Participating in a conference, workshop, ...
Research output
- 1 Poster
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Peptide fraction identification by SE-HPLC and LC-MS/MS analysis of the body mucus from Portugal coastal fish Halobatrachus didactylus
Cunha, M., Coscueta, E. R., Brassesco, M. E., Almada, F., Gonçalves, D. & Pintado, M., Dec 2022.Research output: Contribution to conference › Poster
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