TY - JOUR
T1 - ParticleStats
T2 - open source software for the analysis of particle motility and cytoskeletal polarity
AU - Hamilton, Russell S.
AU - Parton, Richard M.
AU - Oliveira, Raquel A.
AU - Vendra, Georgia
AU - Ball, Graeme
AU - Nasmyth, Kim
AU - Davis, Ilan
N1 - Funding Information:
Wellcome Trust Senior Research Fellowship (081858 to I.D., R.S.H., R.M.P.); Fundac¸ ão para Ciência e a Tecnologia of Portugal fellowship (to R.A.O.); Engineering and Physical Sciences Research Council (to G.B.); State Scholarship Foundation of Greece (to G.V.); Medical Research Council and Wellcome Trust (to K.N.). Funding for open access charge: Wellcome Trust Senior Research Fellowship (to I.D.).
PY - 2010/6/11
Y1 - 2010/6/11
N2 - The study of dynamic cellular processes in living cells is central to biology and is particularly powerful when the motility characteristics of indi-vidual objects within cells can be determined and analysed statistically. However, commercial programs only offer a limited range of inflexible analysis modules and there are currently no open source programs for extensive analysis of particle motility. Here, we describe ParticleStats (http://www.ParticleStats.com), a web server and open source programs, which input the X,Y coordinate positions of objects in time, and output novel analyses, graphical plots and statistics for motile objects. ParticleStats comprises three separate analysis programs. First, ParticleStats: Directionality for the global analysis of polarity, for example microtubule plus end growth in Drosophila oocytes. Second, ParticleStats:Compare for the analysis of saltatory movement in terms of runs and pauses. This can be applied to chromosome segregation and molecular motor-based move-ments. Thirdly ParticleStats:Kymographs for the analysis of kymograph images, for example as applied to separation of chromosomes in mitosis. These analyses have provided key insights into mo-lecular mechanisms that are not possible from qualitative analysis alone and are widely applicable to many other cell biology problems.
AB - The study of dynamic cellular processes in living cells is central to biology and is particularly powerful when the motility characteristics of indi-vidual objects within cells can be determined and analysed statistically. However, commercial programs only offer a limited range of inflexible analysis modules and there are currently no open source programs for extensive analysis of particle motility. Here, we describe ParticleStats (http://www.ParticleStats.com), a web server and open source programs, which input the X,Y coordinate positions of objects in time, and output novel analyses, graphical plots and statistics for motile objects. ParticleStats comprises three separate analysis programs. First, ParticleStats: Directionality for the global analysis of polarity, for example microtubule plus end growth in Drosophila oocytes. Second, ParticleStats:Compare for the analysis of saltatory movement in terms of runs and pauses. This can be applied to chromosome segregation and molecular motor-based move-ments. Thirdly ParticleStats:Kymographs for the analysis of kymograph images, for example as applied to separation of chromosomes in mitosis. These analyses have provided key insights into mo-lecular mechanisms that are not possible from qualitative analysis alone and are widely applicable to many other cell biology problems.
UR - http://www.scopus.com/inward/record.url?scp=77954250305&partnerID=8YFLogxK
U2 - 10.1093/nar/gkq542
DO - 10.1093/nar/gkq542
M3 - Article
C2 - 20542911
AN - SCOPUS:77954250305
SN - 0305-1048
VL - 38
SP - W641-W646
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - SUPPL. 2
M1 - gkq542
ER -