TY - JOUR
T1 - Pedobacter lusitanus sp. nov., isolated from sludge of a deactivated uranium mine
AU - Covas, Cláudia
AU - Caetano, Tânia
AU - Cruz, Andreia
AU - Santos, Tiago
AU - Dias, Liliana
AU - Klein, Guenter
AU - Abdulmawjood, Amir
AU - Rodríguez-Alcalá, Luis M.
AU - Pimentel, Lígia L.
AU - Gomes, Ana
AU - Freitas, Ana Cristina
AU - Garcia-Serrano, Alba
AU - Fontecha, Javier
AU - Mendo, Sónia
N1 - Funding Information:
This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within the project UID/AMB/50017/2013. T. C. and C. C. were supported by FCT, POPH, and European Union grants SFRH/BPD/77900/2011 and SFRH/BD/98446/2013, respectively. A. C. was supported by a postdoctoral grant (BPD/UI88/2886/2013) from the project ‘Sustainable Use of Marine Resources’—MARES (CENTRO-07-ST24-FEDER- 002033), funded by QREN, Mais Centro-Programa Operacional Regional do Centro e União Europeia/Fundo Europeu de Desenvolvimento Regional.
Publisher Copyright:
© 2017 IUMS.
PY - 2017/5
Y1 - 2017/5
N2 - Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16: 1ω7c, C14: 1ω5c, C4: 0, iso-C17: 0, iso-C17: 0 3-OH, C16: 0, anteiso-C15: 0 and iso- C15: 0 3-OH. The main lipids present were phospholipids (60%) and sphingolipids (35%). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA–DNA hybridization similarities between strain NL19T and Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T were 15.3, 16.2, 11.5 and 16.0%, respectively. Strain NL19T can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19T represents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19T (=LMG 29220T=CECT 9028T). An amended description of Pedobacter himalayensis is also included.
AB - Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16: 1ω7c, C14: 1ω5c, C4: 0, iso-C17: 0, iso-C17: 0 3-OH, C16: 0, anteiso-C15: 0 and iso- C15: 0 3-OH. The main lipids present were phospholipids (60%) and sphingolipids (35%). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA–DNA hybridization similarities between strain NL19T and Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T were 15.3, 16.2, 11.5 and 16.0%, respectively. Strain NL19T can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19T represents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19T (=LMG 29220T=CECT 9028T). An amended description of Pedobacter himalayensis is also included.
KW - 16S rRNA gene
KW - Antibacterial activity
KW - GyrB
KW - Intergenic transcribed spacer
KW - Phospholipids and sphingolipids
KW - Polyphasic taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85020441779&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001814
DO - 10.1099/ijsem.0.001814
M3 - Article
C2 - 28109203
AN - SCOPUS:85020441779
SN - 1466-5026
VL - 67
SP - 1339
EP - 1348
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 5
M1 - 001814
ER -