Peptide synthesis catalyzed by Cynara cardunculus L. Acid protease in aqueous-organic biphasic systems

Marlene T. Barros; Carlos J. Faro; Euclides M. V. Pires

doi:10.1007/BF00138558

Peptide synthesis catalyzed by Cynara cardunculus L. Acid protease in aqueous-organic biphasic systems

Marlene T. Barros^*, Carlos J. Faro, Euclides M. V. Pires

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The ability of Cynara cardunculus L. protease to perform peptide synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products were separated by RP-HPLC and identified by amino acid analysis and by EI-MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amount produced by pepsin under the same conditions.

Original language	English
Pages (from-to)	653-656
Number of pages	4
Journal	Biotechnology Letters
Volume	15
Issue number	6
DOIs	https://doi.org/10.1007/BF00138558
Publication status	Published - Jun 1993
Externally published	Yes

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title = "Peptide synthesis catalyzed by Cynara cardunculus L. Acid protease in aqueous-organic biphasic systems",

abstract = "The ability of Cynara cardunculus L. protease to perform peptide synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products were separated by RP-HPLC and identified by amino acid analysis and by EI-MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amount produced by pepsin under the same conditions.",

author = "Barros, {Marlene T.} and Faro, {Carlos J.} and Pires, {Euclides M. V.}",

year = "1993",

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AU - Barros, Marlene T.

AU - Faro, Carlos J.

AU - Pires, Euclides M. V.

PY - 1993/6

Y1 - 1993/6

N2 - The ability of Cynara cardunculus L. protease to perform peptide synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products were separated by RP-HPLC and identified by amino acid analysis and by EI-MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amount produced by pepsin under the same conditions.

AB - The ability of Cynara cardunculus L. protease to perform peptide synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products were separated by RP-HPLC and identified by amino acid analysis and by EI-MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amount produced by pepsin under the same conditions.

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DO - 10.1007/BF00138558

M3 - Article

AN - SCOPUS:0027291567

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SP - 653

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 6

ER -

Peptide synthesis catalyzed by Cynara cardunculus L. Acid protease in aqueous-organic biphasic systems

Abstract

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