Performance of quercus suber L. at nursery stage: application of two bio-inoculants under two distinct environments

Giovânia C. Araújo; Nadine R. Sousa; Miguel A. Ramos; Alberto L. Vega; Paula M. L. Castro

doi:10.1007/s13595-018-0700-3

Performance of quercus suber L. at nursery stage: application of two bio-inoculants under two distinct environments

Giovânia C. Araújo, Nadine R. Sousa, Miguel A. Ramos, Alberto L. Vega, Paula M. L. Castro^*

^*Corresponding author for this work

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Abstract

Key message: Despite the fact that the technique of application of bioinoculants improved the quality ofQuercus suberL. seedlings produced in nurseries, these benefits are dependent on the ecological conditions of the site and the composition of the applied inoculum, which interferes with the profile of the local fungal community. Context: Quercus suber L. plays a key ecological and socio-economical role in the Iberian Peninsula. Symbiotic ectomycorrhizal fungi-ECM are crucial partners of several tree species, and assessing the efficacy of bioinoculants at nursery stage helps devising tools to increase plant resilience. Aims: The aim of this study was to compare the effects of two inocula formulations of mixed ECM fungi and bacteria on the quality of seedlings produced in two forest nurseries, differing in environmental conditions and forest embedment. Methods: Quercus suber L. seedlings were inoculated with a commercial product containing Pisolithus tinctorius (Pers) Coker & Couch, Scleroderma sp., and six bacterial species and with a non-commercial fungal and bacterial dual inoculum (Suillus granulatus (L.) Roussel + Mesorhizobium sp.). Biometric and nutritional parameters and morphological quality indexes were determined on seedlings. The ECM community was assessed by denaturing gradient gel electrophoresis and cloning-sequencing. Results: In both nurseries, the seedling quality index in inoculated was up to 2-fold higher than in non-inoculated seedlings. Plant biomass differed significantly among nurseries. The inoculum influenced the profile of the fungal community. S. granulatus and P. tinctorius persisted for 6 months in the inoculated seedlings. Conclusion: The nursery ecosystem influenced plant growth. Inoculation treatments increased plant performance; however, the dual inoculum resulted in more consistent improvements of Q. suber at nursery stage, highlighting the importance of inocula selection.

Original language	English
Article number	29
Pages (from-to)	1-12
Number of pages	12
Journal	Annals of Forest Science
Volume	75
Issue number	1
DOIs	https://doi.org/10.1007/s13595-018-0700-3
Publication status	Published - 1 Mar 2018

Keywords

Dickson Quality Index
Ectomycorrhizal fungi
Oak
PGPB
Suillus granulatus

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10.1007/s13595-018-0700-3Licence: CC BY

19808183Final published version, 994 KBLicence: CC BY

http://hdl.handle.net/10400.14/25943Licence: CC BY

Cite this

@article{afad38057a4046598bcbd173bf96c5df,

title = "Performance of quercus suber L. at nursery stage: application of two bio-inoculants under two distinct environments",

abstract = "Key message: Despite the fact that the technique of application of bioinoculants improved the quality ofQuercus suberL. seedlings produced in nurseries, these benefits are dependent on the ecological conditions of the site and the composition of the applied inoculum, which interferes with the profile of the local fungal community. Context: Quercus suber L. plays a key ecological and socio-economical role in the Iberian Peninsula. Symbiotic ectomycorrhizal fungi-ECM are crucial partners of several tree species, and assessing the efficacy of bioinoculants at nursery stage helps devising tools to increase plant resilience. Aims: The aim of this study was to compare the effects of two inocula formulations of mixed ECM fungi and bacteria on the quality of seedlings produced in two forest nurseries, differing in environmental conditions and forest embedment. Methods: Quercus suber L. seedlings were inoculated with a commercial product containing Pisolithus tinctorius (Pers) Coker & Couch, Scleroderma sp., and six bacterial species and with a non-commercial fungal and bacterial dual inoculum (Suillus granulatus (L.) Roussel + Mesorhizobium sp.). Biometric and nutritional parameters and morphological quality indexes were determined on seedlings. The ECM community was assessed by denaturing gradient gel electrophoresis and cloning-sequencing. Results: In both nurseries, the seedling quality index in inoculated was up to 2-fold higher than in non-inoculated seedlings. Plant biomass differed significantly among nurseries. The inoculum influenced the profile of the fungal community. S. granulatus and P. tinctorius persisted for 6 months in the inoculated seedlings. Conclusion: The nursery ecosystem influenced plant growth. Inoculation treatments increased plant performance; however, the dual inoculum resulted in more consistent improvements of Q. suber at nursery stage, highlighting the importance of inocula selection.",

keywords = "Dickson Quality Index, Ectomycorrhizal fungi, Oak, PGPB, Suillus granulatus",

author = "Ara{\'u}jo, {Giov{\^a}nia C.} and Sousa, {Nadine R.} and Ramos, {Miguel A.} and Vega, {Alberto L.} and Castro, {Paula M. L.}",

note = "Funding Information: Funding This work was supported by Amorim Florestal, S.A., and National Funds from Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT) through project UID/Multi/50016/2013. Open access was financed by the project NORTE-01-0246-FEDER-000011, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Funding Information: Acknowledgements Giovania Araujo is grateful for the scholarship supported by CAPES-Brazilian Federal Agency for Support of the Ministry of Education of Brazil. The authors also acknowledge Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia and Fundo Social Europeu (III Quadro Comunit{\'a}rio de Apoio) for the research grants of Nadine R. Sousa (SFRH/BPD/89112/2012) and Miguel Ramos (SFRH/BD/111056/ 2015). The authors would also like to thank Viveiros de Amarante and Viveiros da Mata do Bu{\c c}aco for their technical support. Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

month = mar,

day = "1",

doi = "10.1007/s13595-018-0700-3",

language = "English",

volume = "75",

pages = "1--12",

journal = "Annals of Forest Science",

issn = "1286-4560",

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TY - JOUR

T1 - Performance of quercus suber L. at nursery stage

T2 - application of two bio-inoculants under two distinct environments

AU - Araújo, Giovânia C.

AU - Sousa, Nadine R.

AU - Ramos, Miguel A.

AU - Vega, Alberto L.

AU - Castro, Paula M. L.

N1 - Funding Information: Funding This work was supported by Amorim Florestal, S.A., and National Funds from Fundação para a Ciência e a Tecnologia (FCT) through project UID/Multi/50016/2013. Open access was financed by the project NORTE-01-0246-FEDER-000011, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Funding Information: Acknowledgements Giovania Araujo is grateful for the scholarship supported by CAPES-Brazilian Federal Agency for Support of the Ministry of Education of Brazil. The authors also acknowledge Fundação para a Ciência e a Tecnologia and Fundo Social Europeu (III Quadro Comunitário de Apoio) for the research grants of Nadine R. Sousa (SFRH/BPD/89112/2012) and Miguel Ramos (SFRH/BD/111056/ 2015). The authors would also like to thank Viveiros de Amarante and Viveiros da Mata do Buçaco for their technical support. Publisher Copyright: © 2018, The Author(s).

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Key message: Despite the fact that the technique of application of bioinoculants improved the quality ofQuercus suberL. seedlings produced in nurseries, these benefits are dependent on the ecological conditions of the site and the composition of the applied inoculum, which interferes with the profile of the local fungal community. Context: Quercus suber L. plays a key ecological and socio-economical role in the Iberian Peninsula. Symbiotic ectomycorrhizal fungi-ECM are crucial partners of several tree species, and assessing the efficacy of bioinoculants at nursery stage helps devising tools to increase plant resilience. Aims: The aim of this study was to compare the effects of two inocula formulations of mixed ECM fungi and bacteria on the quality of seedlings produced in two forest nurseries, differing in environmental conditions and forest embedment. Methods: Quercus suber L. seedlings were inoculated with a commercial product containing Pisolithus tinctorius (Pers) Coker & Couch, Scleroderma sp., and six bacterial species and with a non-commercial fungal and bacterial dual inoculum (Suillus granulatus (L.) Roussel + Mesorhizobium sp.). Biometric and nutritional parameters and morphological quality indexes were determined on seedlings. The ECM community was assessed by denaturing gradient gel electrophoresis and cloning-sequencing. Results: In both nurseries, the seedling quality index in inoculated was up to 2-fold higher than in non-inoculated seedlings. Plant biomass differed significantly among nurseries. The inoculum influenced the profile of the fungal community. S. granulatus and P. tinctorius persisted for 6 months in the inoculated seedlings. Conclusion: The nursery ecosystem influenced plant growth. Inoculation treatments increased plant performance; however, the dual inoculum resulted in more consistent improvements of Q. suber at nursery stage, highlighting the importance of inocula selection.

AB - Key message: Despite the fact that the technique of application of bioinoculants improved the quality ofQuercus suberL. seedlings produced in nurseries, these benefits are dependent on the ecological conditions of the site and the composition of the applied inoculum, which interferes with the profile of the local fungal community. Context: Quercus suber L. plays a key ecological and socio-economical role in the Iberian Peninsula. Symbiotic ectomycorrhizal fungi-ECM are crucial partners of several tree species, and assessing the efficacy of bioinoculants at nursery stage helps devising tools to increase plant resilience. Aims: The aim of this study was to compare the effects of two inocula formulations of mixed ECM fungi and bacteria on the quality of seedlings produced in two forest nurseries, differing in environmental conditions and forest embedment. Methods: Quercus suber L. seedlings were inoculated with a commercial product containing Pisolithus tinctorius (Pers) Coker & Couch, Scleroderma sp., and six bacterial species and with a non-commercial fungal and bacterial dual inoculum (Suillus granulatus (L.) Roussel + Mesorhizobium sp.). Biometric and nutritional parameters and morphological quality indexes were determined on seedlings. The ECM community was assessed by denaturing gradient gel electrophoresis and cloning-sequencing. Results: In both nurseries, the seedling quality index in inoculated was up to 2-fold higher than in non-inoculated seedlings. Plant biomass differed significantly among nurseries. The inoculum influenced the profile of the fungal community. S. granulatus and P. tinctorius persisted for 6 months in the inoculated seedlings. Conclusion: The nursery ecosystem influenced plant growth. Inoculation treatments increased plant performance; however, the dual inoculum resulted in more consistent improvements of Q. suber at nursery stage, highlighting the importance of inocula selection.

KW - Dickson Quality Index

KW - Ectomycorrhizal fungi

KW - Oak

KW - PGPB

KW - Suillus granulatus

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DO - 10.1007/s13595-018-0700-3

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AN - SCOPUS:85043349663

SN - 1286-4560

VL - 75

SP - 1

EP - 12

JO - Annals of Forest Science

JF - Annals of Forest Science

IS - 1

M1 - 29

ER -

Performance of quercus suber L. at nursery stage: application of two bio-inoculants under two distinct environments

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Performance of quercus suber L. at nursery stage: application of two bio-inoculants under two distinct environments

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