TY - JOUR
T1 - Phenolic composition and wound healing potential assessment of Moroccan Henna (Lawsonia inermis) aqueous extracts
AU - Massoudi, Soukaina El
AU - Zinedine, Abdellah
AU - Rocha, João Miguel
AU - Benidir, Meryem
AU - Najjari, Ilham
AU - Ghadraoui, Lahsen El
AU - Benjelloun, Meryem
AU - Errachidi, Faouzi
N1 - Funding Information:
The research was supported by Functional Ecology and Environmental Engineering Laboratory funds of the Sidi Mohammed Ben Abdellah University, Morocco.
Funding Information:
The authors would like to acknowledge the COST Action 18101 SOURDOMICS—Sourdough biotechnology network towards novel, healthier and sustainable food and bioprocesses ( https://sourdomics.com/ ; https://www.cost.eu/actions/CA18101/ , accessed on 15 May 2023), where the author Z.A. is member of the working groups 2, 3, 4, 5, 7 and 8, and the author J.M.R. is the Chair and Grant Holder Scientific Representative and is supported by COST (European Co-operation in Science and Technology) ( https://www.cost.eu/ , accessed on 25 May 2023). COST is a funding agency for research and innovation networks. Author J.M.R. also acknowledges the Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal, as well as the support made by LA/P/0045/2020 (ALiCE) and UIDB/00511/2020-UIDP/00511/2020 (LEPABE) funded by national funds through FCT/MCTES (PIDDAC).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/6/15
Y1 - 2023/6/15
N2 - The present study aims at valorizing Moroccan Henna (Lawsonia inermis) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of Henna leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of L. inermis to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in Henna leaf extracts from Alnif, while Tazarine Henna aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (Henna coloring agent) are higher in Henna collected from Alnif. Aqueous Henna leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with Henna extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied Henna-based formulations. These findings suggest the interest of potential development of Henna-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.
AB - The present study aims at valorizing Moroccan Henna (Lawsonia inermis) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of Henna leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of L. inermis to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in Henna leaf extracts from Alnif, while Tazarine Henna aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (Henna coloring agent) are higher in Henna collected from Alnif. Aqueous Henna leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with Henna extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied Henna-based formulations. These findings suggest the interest of potential development of Henna-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.
KW - Henna
KW - Lawsonia inermis
KW - Morocco
KW - Leaves
KW - Phytochemical
KW - Wound healing activity
UR - http://www.scopus.com/inward/record.url?scp=85163751142&partnerID=8YFLogxK
U2 - 10.3390/cosmetics10030092
DO - 10.3390/cosmetics10030092
M3 - Article
SN - 2079-9284
VL - 10
JO - Cosmetics
JF - Cosmetics
IS - 3
M1 - 92
ER -