Photodynamic effect of glycochlorin conjugates in human cancer epithelial cells

A. T.P.C. Gomes; M. A.F. Faustino; M. G.P.M.S. Neves; V. F. Ferreira; A. Juarranz; J. A.S. Cavaleiro; F. Sanz-Rodríguez

doi:10.1039/c5ra04345j

Photodynamic effect of glycochlorin conjugates in human cancer epithelial cells

A. T.P.C. Gomes, M. A.F. Faustino, M. G.P.M.S. Neves^*, V. F. Ferreira, A. Juarranz, J. A.S. Cavaleiro, F. Sanz-Rodríguez

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The photodynamic effect of glycochlorins 1-4 was evaluated for the first time in human epithelial cells (HeLa) and their PDT efficiency was compared with the results obtained when using human normal skin keratinocytes cells (HaCaT). It was demonstrated that the galactose conjugate is the only photosensitizer that is selective for HeLa cells, without causing a significant effect on the HaCaT cells. Morphological studies carried out after the photodynamic treatment attested such selectivity and showed that HeLa cells suffer a strong cytoplasmic vacuolization and a pronounced retraction, while in HaCaT cells the damage, under the same protocol conditions, is scarce.

Original language	English
Pages (from-to)	33496-33502
Number of pages	7
Journal	RSC Advances
Volume	5
Issue number	42
DOIs	https://doi.org/10.1039/c5ra04345j
Publication status	Published - 2015
Externally published	Yes

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title = "Photodynamic effect of glycochlorin conjugates in human cancer epithelial cells",

abstract = "The photodynamic effect of glycochlorins 1-4 was evaluated for the first time in human epithelial cells (HeLa) and their PDT efficiency was compared with the results obtained when using human normal skin keratinocytes cells (HaCaT). It was demonstrated that the galactose conjugate is the only photosensitizer that is selective for HeLa cells, without causing a significant effect on the HaCaT cells. Morphological studies carried out after the photodynamic treatment attested such selectivity and showed that HeLa cells suffer a strong cytoplasmic vacuolization and a pronounced retraction, while in HaCaT cells the damage, under the same protocol conditions, is scarce.",

author = "Gomes, {A. T.P.C.} and Faustino, {M. A.F.} and Neves, {M. G.P.M.S.} and Ferreira, {V. F.} and A. Juarranz and Cavaleiro, {J. A.S.} and F. Sanz-Rodr{\'i}guez",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2015.",

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doi = "10.1039/c5ra04345j",

language = "English",

volume = "5",

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T1 - Photodynamic effect of glycochlorin conjugates in human cancer epithelial cells

AU - Gomes, A. T.P.C.

AU - Faustino, M. A.F.

AU - Neves, M. G.P.M.S.

AU - Ferreira, V. F.

AU - Juarranz, A.

AU - Cavaleiro, J. A.S.

AU - Sanz-Rodríguez, F.

PY - 2015

Y1 - 2015

N2 - The photodynamic effect of glycochlorins 1-4 was evaluated for the first time in human epithelial cells (HeLa) and their PDT efficiency was compared with the results obtained when using human normal skin keratinocytes cells (HaCaT). It was demonstrated that the galactose conjugate is the only photosensitizer that is selective for HeLa cells, without causing a significant effect on the HaCaT cells. Morphological studies carried out after the photodynamic treatment attested such selectivity and showed that HeLa cells suffer a strong cytoplasmic vacuolization and a pronounced retraction, while in HaCaT cells the damage, under the same protocol conditions, is scarce.

AB - The photodynamic effect of glycochlorins 1-4 was evaluated for the first time in human epithelial cells (HeLa) and their PDT efficiency was compared with the results obtained when using human normal skin keratinocytes cells (HaCaT). It was demonstrated that the galactose conjugate is the only photosensitizer that is selective for HeLa cells, without causing a significant effect on the HaCaT cells. Morphological studies carried out after the photodynamic treatment attested such selectivity and showed that HeLa cells suffer a strong cytoplasmic vacuolization and a pronounced retraction, while in HaCaT cells the damage, under the same protocol conditions, is scarce.

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DO - 10.1039/c5ra04345j

M3 - Article

AN - SCOPUS:84928036893

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VL - 5

SP - 33496

EP - 33502

JO - RSC Advances

JF - RSC Advances

IS - 42

ER -

Photodynamic effect of glycochlorin conjugates in human cancer epithelial cells

Abstract

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