TY - JOUR
T1 - Photodynamic inactivation of methicillin-resistant Staphylococcus aureus on skin using a porphyrinic formulation
AU - Braz, Márcia
AU - Salvador, Diana
AU - Gomes, Ana T.P.C.
AU - Mesquita, Mariana Q.
AU - Faustino, M. Amparo F.
AU - Neves, M. Graça P.M.S.
AU - Almeida, Adelaide
N1 - Funding Information:
Thanks are due to the University of Aveiro, to FCT/MEC for the financial support to CESAM (UID/AMB/50017/2019 and UIDB/50017/2020 + UIDP/50017/2020), QOPNA (FCT UID/QUI/00062/2019) and to the LAQV-REQUIMTE (UIDB/50006/2020) research units, to the FCT and to the FCT projects PREVINE (FCT-PTDC/ASPPES/29576/2017), PTDC/QUI-QOR/31770/2017 and P2020-PTDC/QEQ-SUP/5355/2014, through national funds and the co-funding by the F EDER-Operational Thematic Program for Competitiveness and Internationalization−COMPETE 2020 , within the PT2020 Partnership Agreement and also to the Portuguese NMR Network. MM thanks to FCT for her doctoral (SFRH/BD/112517/2015) grant.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/6
Y1 - 2020/6
N2 - Staphylococcus aureus is responsible for skin and soft tissue infections. Having in mind increased antibiotic resistance, in this study the efficacy of antimicrobial photodynamic therapy (aPDT) with a porphyrinic formulation (FORM) as photosensitizer (PS) to photoinactivate methicillin-resistant Staphylococcus aureus (MRSA) on skin was evaluated. Potassium iodide (KI) and iodopovidone (PVP-I) were also tested in combination with FORM as potentiator agents of FORM efficacy. The aPDT protocol was first developed in Phosphate Buffered Saline (PBS, in vitro). Porcine skin was artificially contaminated with MRSA (ex vivo) and treated with FORM, FORM + KI or FORM + PVP-I under white light. The in vitro results showed that FORM was effective to inactivate MRSA. A substantial reduction in the irradiation time, when compared to FORM alone, was observed for FORM + KI and FORM + PVP-I combinations. On skin, reductions in MRSA survival of 3.1 Log10 colony forming units (CFU) mL−1 were observed with FORM at 50 μM. Although the combined action of FORM + KI and FORM + PVP-I potentiated the aPDT efficacy in vitro, this was not observed ex vivo. Overall, the results showed that aPDT using FORM, even without coadjutants, is a promising approach for MRSA inactivation on skin.
AB - Staphylococcus aureus is responsible for skin and soft tissue infections. Having in mind increased antibiotic resistance, in this study the efficacy of antimicrobial photodynamic therapy (aPDT) with a porphyrinic formulation (FORM) as photosensitizer (PS) to photoinactivate methicillin-resistant Staphylococcus aureus (MRSA) on skin was evaluated. Potassium iodide (KI) and iodopovidone (PVP-I) were also tested in combination with FORM as potentiator agents of FORM efficacy. The aPDT protocol was first developed in Phosphate Buffered Saline (PBS, in vitro). Porcine skin was artificially contaminated with MRSA (ex vivo) and treated with FORM, FORM + KI or FORM + PVP-I under white light. The in vitro results showed that FORM was effective to inactivate MRSA. A substantial reduction in the irradiation time, when compared to FORM alone, was observed for FORM + KI and FORM + PVP-I combinations. On skin, reductions in MRSA survival of 3.1 Log10 colony forming units (CFU) mL−1 were observed with FORM at 50 μM. Although the combined action of FORM + KI and FORM + PVP-I potentiated the aPDT efficacy in vitro, this was not observed ex vivo. Overall, the results showed that aPDT using FORM, even without coadjutants, is a promising approach for MRSA inactivation on skin.
KW - Antimicrobial photodynamic therapy
KW - Iodopovidone
KW - Methicillin-resistant Staphylococcus aureus
KW - Porphyrinic formulation
KW - Potassium iodide
KW - Potentiator agents
UR - http://www.scopus.com/inward/record.url?scp=85084349458&partnerID=8YFLogxK
U2 - 10.1016/j.pdpdt.2020.101754
DO - 10.1016/j.pdpdt.2020.101754
M3 - Article
C2 - 32335190
AN - SCOPUS:85084349458
SN - 1572-1000
VL - 30
JO - Photodiagnosis and Photodynamic Therapy
JF - Photodiagnosis and Photodynamic Therapy
M1 - 101754
ER -
