Photodynamic inactivation of phage Phi6 as SARS-CoV-2 model in wastewater disinfection: effectivity and safety

Maria Bartolomeu; Cátia Vieira; Marta Gomes; Ana T. P. C. Gomes; Maria do Amparo F. Faustino; Maria Graça P. M. S. Neves; Adelaide Almeida

doi:10.3390/eca2022-12707

Photodynamic inactivation of phage Phi6 as SARS-CoV-2 model in wastewater disinfection: effectivity and safety

Maria Bartolomeu, Cátia Vieira, Marta Gomes, Ana T. P. C. Gomes, Maria do Amparo F. Faustino^*, Maria Graça P. M. S. Neves, Adelaide Almeida^*

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

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Abstract

The past 2 years have been marked by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This virus is found in the intestinal tract and reaches the wastewater system, and, consequently, the natural receiving water bodies, and inappropriate or/and inefficient WW treatment is a means of contamination. In the present work, we used a SARS-CoV-2 model—the phage Phi6—to evaluate its survival under different environmental conditions (pH, temperature, salinity, solar, and UV-B irradiation). Then, we tested the efficiency of photodynamic inactivation (PDI) as a WW disinfection alternative method, and, additionally, the impact on the cultivable native marine microorganisms of the PDI-treated WW was evaluated.

Original language	English
Article number	9
Number of pages	8
Journal	Medical Sciences Forum
Volume	12
Issue number	1
DOIs	https://doi.org/10.3390/eca2022-12707
Publication status	Published - 15 Jun 2022
Event	2nd International Electronic Conference on Antibiotics: Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance - Duration: 15 Jun 2022 → 30 Jun 2022

Keywords

Photodynamic inactivation
Environmental factors
Porphyrins
Wastewater
SARS-CoV-2 model
Phage Phi6
Coronavirus
Viruses

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/eca2022-12707Licence: CC BY

52189096Final published version, 2.49 MBLicence: CC BY

http://hdl.handle.net/10400.14/39030Licence: CC BY

CIIS: Center for Interdisciplinary Research in Health
Barros, M. (PI), Rosa, N. (Researcher), Correia, M. J. (Researcher), Caldas, A. C. (Researcher), Amado, J. C. (Researcher), Figueiredo, A. S. (Researcher), Esteves, A. C. (Researcher), Mineiro, A. (Researcher), Abreu, A. M. (Researcher), Duarte, A. S. (Researcher), Almeida, S. F. (Researcher), Correia, A. (Researcher), Moura, A. (Researcher), Almeida, A. (Researcher), Araújo, B. (Researcher), Moura-Netto, C. (Researcher), Ferrito, C. (Researcher), Pais-Vieira, C. (Researcher), Festas, C. (Researcher), Marques-Vieira, C. (Researcher), Catré, D. (Researcher), Nunes, E. (Researcher), Jesus, É. (Researcher), Ribeiro, F. (Researcher), Rosário, F. (Researcher), Fernandes, G. (Researcher), Rato, J. R. (Scholarship holder), Salgado, J. R. (Researcher), Neves-Amado, J. (Researcher), Amendoeira, J. (Researcher), Sá, L. (Researcher), Capelas, M. L. (Researcher), Vieira, M. M. (Researcher), Nunes, M. V. S. (Researcher), Cardoso, M. (Researcher), Veiga, N. J. (Researcher), Fonseca, P. (Researcher), Correia, P. N. (Researcher), Couto, P. (Researcher), Pontífice-Sousa, P. (Researcher), Ravasco, P. (Researcher), Carvalho, P. V. D. (Researcher), Alves, P. (Researcher), Melo, P. (Researcher), Silva, R. (Researcher), Canaipa, R. (Researcher), Noites, R. (Researcher), Rio, R. (Researcher), Almeida, S. (Researcher), Deodato, S. (Researcher), Caldeira, S. (Researcher), Silva, S. (Researcher), Borges, T. (Researcher), Silva, V. (Researcher), Charepe, Z. (Researcher), Rodrigues-Pires, F. (Volunteer), Veludo, F. (Researcher), Carmo, H. (Scholarship holder), Romeiro, J. (Student), Melo, M. (Researcher), Braga, M. (Researcher), Amaral, T. (Researcher), Moreira, M. A. (Researcher), Guerra, N. (Student), Santos, P. (Researcher), Paço, S. (Student), Lynce, S. (Scholarship holder), Miguel, S. (Student), Costa, T. (Researcher), Silva-Neves, V. (Researcher), Silva, A. (Researcher), Carvalho, A. R. (Researcher), Almeida, B. (Researcher), Figueiredo, C. (Researcher), Esteves, E. (Scholarship holder), Araújo, F. M. (Researcher), Garcia, J. G. (Researcher), Santos, L. (Researcher), Santos, N. M. D. (Researcher), Lopes, P. (Researcher), Bornes, R. (Researcher), Silva, R. (Researcher), Costa, S. (Researcher), Silva, S. M. (Researcher), Marques, T. (Researcher), Almeida, A. (Researcher), Santos, M. (Scholarship holder), Santos, P. (Researcher), Miguel, S. (Researcher), Mendes, K. (Researcher), Gomes, A. P. (Researcher), Henriques, J. M. P. (Researcher), Costa, H. A. F. P. (Researcher) & Ribeiro, P. (Researcher)
Fundação para a Ciência e a Tecnologia
1/01/20 → 31/12/24
Project: Research

Cite this

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title = "Photodynamic inactivation of phage Phi6 as SARS-CoV-2 model in wastewater disinfection: effectivity and safety",

abstract = "The past 2 years have been marked by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This virus is found in the intestinal tract and reaches the wastewater system, and, consequently, the natural receiving water bodies, and inappropriate or/and inefficient WW treatment is a means of contamination. In the present work, we used a SARS-CoV-2 model—the phage Phi6—to evaluate its survival under different environmental conditions (pH, temperature, salinity, solar, and UV-B irradiation). Then, we tested the efficiency of photodynamic inactivation (PDI) as a WW disinfection alternative method, and, additionally, the impact on the cultivable native marine microorganisms of the PDI-treated WW was evaluated.",

keywords = "Photodynamic inactivation, Environmental factors, Porphyrins, Wastewater, SARS-CoV-2 model, Phage Phi6, Coronavirus, Viruses",

author = "Maria Bartolomeu and C{\'a}tia Vieira and Marta Gomes and Gomes, {Ana T. P. C.} and Faustino, {Maria do Amparo F.} and Neves, {Maria Gra{\c c}a P. M. S.} and Adelaide Almeida",

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month = jun,

day = "15",

doi = "10.3390/eca2022-12707",

language = "English",

volume = "12",

journal = "Medical Sciences Forum",

issn = "2673-9992",

publisher = "MDPI",

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TY - JOUR

T1 - Photodynamic inactivation of phage Phi6 as SARS-CoV-2 model in wastewater disinfection

T2 - 2nd International Electronic Conference on Antibiotics: Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance

AU - Bartolomeu, Maria

AU - Vieira, Cátia

AU - Gomes, Marta

AU - Gomes, Ana T. P. C.

AU - Faustino, Maria do Amparo F.

AU - Neves, Maria Graça P. M. S.

AU - Almeida, Adelaide

PY - 2022/6/15

Y1 - 2022/6/15

N2 - The past 2 years have been marked by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This virus is found in the intestinal tract and reaches the wastewater system, and, consequently, the natural receiving water bodies, and inappropriate or/and inefficient WW treatment is a means of contamination. In the present work, we used a SARS-CoV-2 model—the phage Phi6—to evaluate its survival under different environmental conditions (pH, temperature, salinity, solar, and UV-B irradiation). Then, we tested the efficiency of photodynamic inactivation (PDI) as a WW disinfection alternative method, and, additionally, the impact on the cultivable native marine microorganisms of the PDI-treated WW was evaluated.

AB - The past 2 years have been marked by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This virus is found in the intestinal tract and reaches the wastewater system, and, consequently, the natural receiving water bodies, and inappropriate or/and inefficient WW treatment is a means of contamination. In the present work, we used a SARS-CoV-2 model—the phage Phi6—to evaluate its survival under different environmental conditions (pH, temperature, salinity, solar, and UV-B irradiation). Then, we tested the efficiency of photodynamic inactivation (PDI) as a WW disinfection alternative method, and, additionally, the impact on the cultivable native marine microorganisms of the PDI-treated WW was evaluated.

KW - Photodynamic inactivation

KW - Environmental factors

KW - Porphyrins

KW - Wastewater

KW - SARS-CoV-2 model

KW - Phage Phi6

KW - Coronavirus

KW - Viruses

U2 - 10.3390/eca2022-12707

DO - 10.3390/eca2022-12707

M3 - Conference article

SN - 2673-9992

VL - 12

JO - Medical Sciences Forum

JF - Medical Sciences Forum

IS - 1

M1 - 9

Y2 - 15 June 2022 through 30 June 2022

ER -

Photodynamic inactivation of phage Phi6 as SARS-CoV-2 model in wastewater disinfection: effectivity and safety

Abstract

Keywords

UN SDGs

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CIIS: Center for Interdisciplinary Research in Health

Cite this