TY - JOUR
T1 - Photoinactivation of bacteria in wastewater by porphyrins
T2 - bacterial β-galactosidase activity and leucine-uptake as methods to monitor the process
AU - Carvalho, Carla M.B.
AU - Gomes, Ana T.P.C.
AU - Fernandes, Sónia C.D.
AU - Prata, Ana C.B.
AU - Almeida, Maria A.
AU - Cunha, Maria A.
AU - Tomé, João P.C.
AU - Faustino, Maria A.F.
AU - Neves, Maria G.P.M.S.
AU - Tomé, Augusto C.
AU - Cavaleiro, José A.S.
AU - Lin, Zhi
AU - Rainho, José P.
AU - Rocha, João
N1 - Funding Information:
Thanks are due to the University of Aveiro, Fundação para a Ciência e a Tecnologia (FCT) and POCI 2010 (FEDER) for funding the Project POCI/CTM/58183/2004. CMB Carvalho, ATPC Gomes and JPC Tomé are also grateful to FCT for their grants.
PY - 2007/9/25
Y1 - 2007/9/25
N2 - The increasing interest in photodynamic antimicrobial chemotherapy as a promising technology for wastewater treatment demands more efficient photosensitizers and faster methods for the monitorization of the photoinactivation process. Two sets of neutral and cationic porphyrins were used as photosensitizers for the photoinactivation of sewage bacteria. Two of the cationic porphyrins revealed to be highly active, even against Gram-negative bacteria, inactivating ca. 94-99.8% of these at 5 μM and after 270 min of irradiation with white light (9 mW cm-2). In addition, two expeditious methods for the evaluation of the bacteria photoinactivation were developed. These methods are based on β-galactosidase activity and on leucine incorporation and give an excellent relation with faecal coliform counts. The evaluation of these two descriptors of bacterial activity, directly related with bacterial abundance, is an easy and fast way to monitor the micro-organisms during the photoinactivation process.
AB - The increasing interest in photodynamic antimicrobial chemotherapy as a promising technology for wastewater treatment demands more efficient photosensitizers and faster methods for the monitorization of the photoinactivation process. Two sets of neutral and cationic porphyrins were used as photosensitizers for the photoinactivation of sewage bacteria. Two of the cationic porphyrins revealed to be highly active, even against Gram-negative bacteria, inactivating ca. 94-99.8% of these at 5 μM and after 270 min of irradiation with white light (9 mW cm-2). In addition, two expeditious methods for the evaluation of the bacteria photoinactivation were developed. These methods are based on β-galactosidase activity and on leucine incorporation and give an excellent relation with faecal coliform counts. The evaluation of these two descriptors of bacterial activity, directly related with bacterial abundance, is an easy and fast way to monitor the micro-organisms during the photoinactivation process.
KW - Faecal coliforms
KW - PACT
KW - PDT
KW - Photoinactivation
KW - Porphyrins
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=34548536322&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2007.04.015
DO - 10.1016/j.jphotobiol.2007.04.015
M3 - Article
C2 - 17616398
AN - SCOPUS:34548536322
SN - 1011-1344
VL - 88
SP - 112
EP - 118
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
IS - 2-3
ER -